Posts Tagged 'primary production'

Interaction between elevated CO2 and phytoplankton-derived organic matter under solar radiation on bacterial metabolism from coastal waters

Microcosm experiments to assess bacterioplankton response to phytoplankton-derived organic matter obtained under current and future-ocean CO2 levels were performed. Surface seawater enriched with inorganic nutrients was bubbled for 8 days with air (current CO2 scenario) or with a 1000 ppm CO2–air mixture (future CO2 scenario) under solar radiation. The organic matter produced under the current and future CO2 scenarios was subsequently used as inoculum. Triplicate 12 L flasks filled with 1.2 µm-filtered natural seawater enriched with the organic matter inocula were incubated in the dark for 8 days under CO2 conditions simulating current and future CO2 scenarios to study the bacterial response. The acidification of the media increased bacterial respiration at the beginning of the experiment while the addition of the organic matter produced under future levels of CO2 was related to changes in bacterial production and abundance. The balance between both, respiration and production, made that the bacteria grown under future CO2 levels with an addition of non-acidified matter showed the best growth efficiency at the end of the incubation. However cells grown under future scenarios with high CO2 levels and acidified organic matter additions did not perform differently than those grown under present CO2 conditions, independently of the addition of acidified or non-acidified organic matter. This study demonstrates that the increase in atmospheric CO2 concentrations can affect bacterioplankton directly by changes in the respiration rate and indirectly by changes on the organic matter with concomitant effects on bacterial production and abundance.

Continue reading ‘Interaction between elevated CO2 and phytoplankton-derived organic matter under solar radiation on bacterial metabolism from coastal waters’

Ocean acidification of a coastal Antarctic marine microbial community reveals a critical threshold for CO2 tolerance in phytoplankton productivity (update)

High-latitude oceans are anticipated to be some of the first regions affected by ocean acidification. Despite this, the effect of ocean acidification on natural communities of Antarctic marine microbes is still not well understood. In this study we exposed an early spring, coastal marine microbial community in Prydz Bay to CO2 levels ranging from ambient (343 µatm) to 1641 µatm in six 650 L minicosms. Productivity assays were performed to identify whether a CO2 threshold existed that led to a change in primary productivity, bacterial productivity, and the accumulation of chlorophyll a (Chl a) and particulate organic matter (POM) in the minicosms. In addition, photophysiological measurements were performed to identify possible mechanisms driving changes in the phytoplankton community. A critical threshold for tolerance to ocean acidification was identified in the phytoplankton community between 953 and 1140 µatm. CO2 levels  ≥ 1140 µatm negatively affected photosynthetic performance and Chl a-normalised primary productivity (csGPP14C), causing significant reductions in gross primary production (GPP14C), Chl a accumulation, nutrient uptake, and POM production. However, there was no effect of CO2 on C : N ratios. Over time, the phytoplankton community acclimated to high CO2 conditions, showing a down-regulation of carbon concentrating mechanisms (CCMs) and likely adjusting other intracellular processes. Bacterial abundance initially increased in CO2 treatments  ≥ 953 µatm (days 3–5), yet gross bacterial production (GBP14C) remained unchanged and cell-specific bacterial productivity (csBP14C) was reduced. Towards the end of the experiment, GBP14C and csBP14C markedly increased across all treatments regardless of CO2 availability. This coincided with increased organic matter availability (POC and PON) combined with improved efficiency of carbon uptake. Changes in phytoplankton community production could have negative effects on the Antarctic food web and the biological pump, resulting in negative feedbacks on anthropogenic CO2 uptake. Increases in bacterial abundance under high CO2 conditions may also increase the efficiency of the microbial loop, resulting in increased organic matter remineralisation and further declines in carbon sequestration.

Continue reading ‘Ocean acidification of a coastal Antarctic marine microbial community reveals a critical threshold for CO2 tolerance in phytoplankton productivity (update)’

Climate change could drive marine food web collapse through altered trophic flows and cyanobacterial proliferation

Global warming and ocean acidification are forecast to exert significant impacts on marine ecosystems worldwide. However, most of these projections are based on ecological proxies or experiments on single species or simplified food webs. How energy fluxes are likely to change in marine food webs in response to future climates remains unclear, hampering forecasts of ecosystem functioning. Using a sophisticated mesocosm experiment, we model energy flows through a species-rich multilevel food web, with live habitats, natural abiotic variability, and the potential for intra- and intergenerational adaptation. We show experimentally that the combined stress of acidification and warming reduced energy flows from the first trophic level (primary producers and detritus) to the second (herbivores), and from the second to the third trophic level (carnivores). Warming in isolation also reduced the energy flow from herbivores to carnivores, the efficiency of energy transfer from primary producers and detritus to herbivores and detritivores, and the living biomass of detritivores, herbivores, and carnivores. Whilst warming and acidification jointly boosted primary producer biomass through an expansion of cyanobacteria, this biomass was converted to detritus rather than to biomass at higher trophic levels—i.e., production was constrained to the base of the food web. In contrast, ocean acidification affected the food web positively by enhancing trophic flow from detritus and primary producers to herbivores, and by increasing the biomass of carnivores. Our results show how future climate change can potentially weaken marine food webs through reduced energy flow to higher trophic levels and a shift towards a more detritus-based system, leading to food web simplification and altered producer–consumer dynamics, both of which have important implications for the structuring of benthic communities.

Continue reading ‘Climate change could drive marine food web collapse through altered trophic flows and cyanobacterial proliferation’

Diatom performance in a future ocean: interactions between nitrogen limitation, temperature, and CO2-induced seawater acidification

Phytoplankton cells living in the surface waters of oceans are experiencing alterations in environmental conditions associated with global change. Given their importance in global primary productivity, it is of considerable concern to know how these organisms will perform physiologically under the changing levels of pH, temperatures, and nutrients predicted for future oceanic ecosystems. Here we show that the model diatom, Thalassiosira pseudonana, when grown at different temperatures (20 or 24 °C), pCO2 (400 or 1000 µatm), and nitrate concentrations (2.5 or 102.5 µmol l−1), displayed contrasting performance in its physiology. Elevated pCO2 (and hence seawater acidification) under the nitrate-limited conditions led to decreases in specific growth rate, cell size, pigment content, photochemical quantum yield of PSII, and photosynthetic carbon fixation. Furthermore, increasing the temperature exacerbated the negative effects of the seawater acidification associated with elevated pCO2 on specific growth rate and chlorophyll content under the N-limited conditions. These results imply that a reduced upward transport of nutrients due to enhanced stratification associated with ocean warming might act synergistically to reduce growth and carbon fixation by diatoms under progressive ocean acidification, with important ramifications for ocean productivity and the strength of the biological CO2 pump.

Continue reading ‘Diatom performance in a future ocean: interactions between nitrogen limitation, temperature, and CO2-induced seawater acidification’

Taking the metabolic pulse of the world’s coral reefs

Worldwide, coral reef ecosystems are experiencing increasing pressure from a variety of anthropogenic perturbations including ocean warming and acidification, increased sedimentation, eutrophication, and overfishing, which could shift reefs to a condition of net calcium carbonate (CaCO3) dissolution and erosion. Herein, we determine the net calcification potential and the relative balance of net organic carbon metabolism (net community production; NCP) and net inorganic carbon metabolism (net community calcification; NCC) within 23 coral reef locations across the globe. In light of these results, we consider the suitability of using these two metrics developed from total alkalinity (TA) and dissolved inorganic carbon (DIC) measurements collected on different spatiotemporal scales to monitor coral reef biogeochemistry under anthropogenic change. All reefs in this study were net calcifying for the majority of observations as inferred from alkalinity depletion relative to offshore, although occasional observations of net dissolution occurred at most locations. However, reefs with lower net calcification potential (i.e., lower TA depletion) could shift towards net dissolution sooner than reefs with a higher potential. The percent influence of organic carbon fluxes on total changes in dissolved inorganic carbon (DIC) (i.e., NCP compared to the sum of NCP and NCC) ranged from 32% to 88% and reflected inherent biogeochemical differences between reefs. Reefs with the largest relative percentage of NCP experienced the largest variability in seawater pH for a given change in DIC, which is directly related to the reefs ability to elevate or suppress local pH relative to the open ocean. This work highlights the value of measuring coral reef carbonate chemistry when evaluating their susceptibility to ongoing global environmental change and offers a baseline from which to guide future conservation efforts aimed at preserving these valuable ecosystems.

Continue reading ‘Taking the metabolic pulse of the world’s coral reefs’

Increased CO2 and iron availability effects on carbon assimilation and calcification on the formation of Emiliania huxleyi blooms in a coastal phytoplankton community

Highlights

• Increased dFe enhanced carbon fixation promoting a bloom of Emiliania huxleyi.
• High CO2 decreased carbon production during a bloom of Emiliania huxleyi.
• Carbon uptake was unaffected by CO2 and Fe availability in a bloom of Emiliania huxleyi.

Abstract

In the present work, we exposed a natural phytoplankton community to either present (390-μatm, LC) or future CO2 levels predicted for year-2100 (900-μatm, HC) combined with ambient (4.5 nmol L−1, −DFB) or high (12 nmol L−1, +DFB) dissolved iron (dFe) levels, during 25 days by using mesocosms. We report on changes in carbon assimilation processes (acquisition, fixation, and calcification) of the phytoplankton community due to increased dissolved CO2 and dFe and to the interaction of both factors. The isotopic disequilibrium assay results showed that inorganic carbon (Ci) acquisition by the community was unaffected by CO2 and Fe availability. The main Ci source for photosynthesis was HCO3 and external carbonic anhydrase activity was only detected at the beginning of the experiment, suggesting a relevant role for HCO3 transporters in the phytoplankton community developed in all treatments. However, there was a significant effect of both factors on particulate organic carbon (POC) content, particulate calcium production and carbon fixation rates. Increased dFe at LC conditions led to the highest values of carbon fixation and POC of all treatments, promoting a massive Emiliania huxleyi bloom. This response was not observed in the HC treatments. The latter indicates a negative impact of increased CO2 on the formation of E. huxleyi blooms, in agreement with the observed significant reduction in calcium production under HC. Our results suggest that ocean acidification can decrease primary production under iron-replete conditions in E. huxleyi blooming areas, affecting the biological carbon pump in coastal ecosystems.

Continue reading ‘Increased CO2 and iron availability effects on carbon assimilation and calcification on the formation of Emiliania huxleyi blooms in a coastal phytoplankton community’

Species interactions can shift the response of a maerl bed community to ocean acidification and warming (update)

Predicted ocean acidification and warming are likely to have major implications for marine organisms, especially marine calcifiers. However, little information is available on the response of marine benthic communities as a whole to predicted changes. Here, we experimentally examined the combined effects of temperature and partial pressure of carbon dioxide (pCO2) increases on the response of maerl bed assemblages, composed of living and dead thalli of the free-living coralline alga Lithothamnion corallioides, epiphytic fleshy algae, and grazer species. Two 3-month experiments were performed in the winter and summer seasons in mesocosms with four different combinations of pCO2 (ambient and high pCO2) and temperature (ambient and +3 °C). The response of maerl assemblages was assessed using metabolic measurements at the species and assemblage scales. This study suggests that seasonal variability represents an important driver influencing the magnitude and the direction of species and community response to climate change. Gross primary production and respiration of assemblages was enhanced by high pCO2 conditions in the summer. This positive effect was attributed to the increase in epiphyte biomass, which benefited from higher CO2 concentrations for growth and primary production. Conversely, high pCO2 drastically decreased the calcification rates in assemblages. This response can be attributed to the decline in calcification rates of living L. corallioides due to acidification and increased dissolution of dead L. corallioides. Future changes in pCO2 and temperature are likely to promote the development of non-calcifying algae to the detriment of the engineer species L. corallioides. The development of fleshy algae may be modulated by the ability of grazers to regulate epiphyte growth. However, our results suggest that predicted changes will negatively affect the metabolism of grazers and potentially their ability to control epiphyte abundance. We show here that the effects of pCO2 and temperature on maerl bed communities were weakened when these factors were combined. This underlines the importance of examining multi-factorial approaches and community-level processes, which integrate species interactions, to better understand the impact of global change on marine ecosystems.

Continue reading ‘Species interactions can shift the response of a maerl bed community to ocean acidification and warming (update)’


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Ocean acidification in the IPCC AR5 WG II

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