Archive for the 'Science' Category

Oyster biomineralisation under ocean acidification: from genes to shell

Biomineralisation is one of the key processes that is notably affected in marine calcifiers such as oysters under ocean acidification (OA). Understanding molecular changes in the biomineralisation process under OA and its heritability, therefore, is key to developing conservation strategies for protecting ecologically and economically important oyster species. To do this, in this study we have explicitly chosen the tissue involved in biomineralisation (mantle) of an estuarine commercial oyster species, Crassostrea hongkongensis. The primary aim of this study is to understand the influence of DNA methylation over gene expression of mantle tissue under decreased ~ pH 7.4, a proxy of OA, and to extrapolate if these molecular changes can be observed in the product of biomineralisation – the shell. We grew early juvenile C. hongkongensis, under decreased ~ pH 7.4 and control ~ pH 8.0 over 4.5 months and studied OA‐induced DNA methylation and gene expression patterns along with shell properties such as microstructure, crystal orientation and hardness.

The population of oysters used in this study was found to be moderately resilient to OA at the end of the experiment. The expression of key biomineralisation related genes such as carbonic anhydrase and alkaline phosphatase remained unaffected, thus, mechanical properties of the shell (shell growth rate, hardness and crystal orientation) were also maintained without any significant difference between control and OA conditions with signs of severe dissolution. In addition, this study makes three major conclusions: 1) higher expression of Ca2+ binding/signalling related genes in the mantle play a key role in maintaining biomineralisation under OA, 2) DNA methylation changes occur in response to OA, however, these methylation changes do not directly control gene expression, 3) OA would be more of a ‘dissolution problem’ rather than a ‘biomineralisation problem’ for resilient species that maintain calcification rate with normal shell growth and mechanical properties.

Continue reading ‘Oyster biomineralisation under ocean acidification: from genes to shell’

Diel transcriptional oscillations of a plastid antiporter reflect increased resilience of Thalassiosira pseudonana in elevated CO2

Acidification of the ocean due to high atmospheric CO2 levels may increase the resilience of diatoms causing dramatic shifts in abiotic and biotic cycles with lasting implications on marine ecosystems. Here, we report a potential bioindicator of a shift in the resilience of a coastal and centric model diatom Thalassiosira pseudonana under elevated CO2. Specifically, we have discovered, through EGFP-tagging, a plastid membrane localized putative Na+(K+)/H+ antiporter that is significantly upregulated at >800 ppm CO2, with a potentially important role in maintaining pH homeostasis. Notably, transcript abundance of this antiporter gene was relatively low and constant over the diel cycle under contemporary CO2 conditions. In future acidified oceanic conditions, dramatic oscillation with >10-fold change between nighttime (high) and daytime (low) transcript abundances of the antiporter was associated with increased resilience of T. pseudonana. By analyzing metatranscriptomic data from the Tara Oceans project, we demonstrate that phylogenetically diverse diatoms express homologs of this antiporter across the globe. We propose that the differential between night- and daytime transcript levels of the antiporter could serve as a bioindicator of a shift in the resilience of diatoms in response to high CO2 conditions in marine environments.

Continue reading ‘Diel transcriptional oscillations of a plastid antiporter reflect increased resilience of Thalassiosira pseudonana in elevated CO2’

Acclimation history of elevated temperature reduces the tolerance of coralline algae to additional acute thermal stress

Increasing atmospheric CO2 is driving major environmental changes in the ocean, such as an increase in average ocean temperature, a decrease in average ocean pH (ocean acidification or OA), and an increase in the number and severity of extreme climatic events (e.g., anomalous temperature events and heatwaves). Uncertainty exists in the capacity for species to withstand these stressors occurring concomitantly. Here, we tested whether an acclimation history of ocean warming (OW) and OA affects the physiological responses of an abundant, reef-building species of crustose coralline algae (CCA), Porolithon cf. onkodes, to chronic and acute thermal stress. To address this, we exposed algae to varying temperature and pH levels for 6 weeks and this chronic treatment experiment was followed by an acute exposure to an anomalous temperature event (+4–6°C from acclimation temperature). Net photosynthetic rate was negatively affected across all treatments by increasing temperature during the acute temperature event, however, algae acclimated to the control temperature were able to maintain photosynthetic rates for +4°C above their acclimation temperature, whereas algae acclimated to elevated temperature were not. Average relative change in O2 produced resulted in a 100–175% decrease, with the largest decrease found in algae acclimated to the combined treatment of elevated temperature and reduced pH. We conclude that acclimation to chronic global change stressors (i.e., OW and OA) will reduce the tolerance of P. cf. onkodes to anomalous increases in temperature, and this may have implications for reef building processes.

Continue reading ‘Acclimation history of elevated temperature reduces the tolerance of coralline algae to additional acute thermal stress’

Exploring coastal acidification and oyster restoration activities on the United States Atlantic coast

Executive Summary

The global ocean mediates the effect of climate change and anthropogenic carbon emissions by absorbing atmospheric carbon dioxide (Ellis et al., 2017). The ocean’s absorption of carbon dioxide results in a change in ocean chemistry and decline in seawater pH known as ocean acidification (Kapsenberg and Cyronak, 2018). Changes in ocean chemistry and pH may also be driven by primary production activity, upwelling, and river runoff into marine environments (Richards et al., 2014). Ocean acidification has the potential to adversely affect numerous marine organisms (Kapsenberg and Cyronak, 2018), however, it can be especially problematic for calcifying shellfish species (Swezey et al., 2020) like the Eastern Oyster and larval or juvenile stage organisms (Mangi et al., 2018). Temperature, salinity, dissolved oxygen levels, and acidification impact the health and longevity of oysters and oyster reefs. Oyster reefs offer numerous ecosystem services. These reefs provide habitat for benthic invertebrates, seabirds and fish that rely on reefs for feeding, nursery, and breeding grounds (Burrows et al., 2005). The Eastern Oyster (Crassostrea virginica) is a native oyster species of the U.S. Atlantic Coast. Although oysters reefs support coastal livelihoods and offer numerous ecosystem services, many reefs have been degraded by anthropogenic activities (Burrows et al., 2005). Pollution, over-harvest, and an increase in loading of suspended sediments are key threats to oyster reef health (Burrows et al., 2005). Oyster reef restoration projects focus on returning reefs to their natural state. Given the role of oysters as ecosystem engineers, and the many benefits that may be derived from healthy oyster reefs, restoration projects are a priority for communities throughout the U.S. Atlantic Coast.

Cooley et al. 2016 recommends several effective community actions that may be taken to help address ocean acidification today. This project focuses on two non-legislative actions discussed by Cooley et al. 2016. These are public education related to coastal acidification and resilience management through oyster reef restoration projects. The purpose of this project is to support coastal resource-reliant communities on the U.S. Atlantic Coast in preparing for the potential future impacts of ocean acidification on C. virginica. The project examines trends in the oyster reef restoration projects presently underway at the state and local level along the U.S. Atlantic Coast, and it considers how coastal acidification may affect the longevity of the region’s oyster reefs. Finally, the project considers the future research and management considerations needed to adequately protect oyster reefs under changing climatic conditions.

Continue reading ‘Exploring coastal acidification and oyster restoration activities on the United States Atlantic coast’

Exposure time modulates the effects of climate change-related stressors on fertile sporophytes and early-life stage performance of a habitat-forming kelp species

Highlight

  • Ocean warming (OW) reduced the sorus photosynthetic performance.
  • OW reduced meiospore germination rate.
  • OW and ocean acidification reduced meiospore release (MR).
  • MR is more sensitive to temperature changes than to pCO2 changes.
  • Longer exposure to OW increased the negative effects on germination rate.

Abstract

Understanding the impact of increases in pCO2 (OA) and extreme changes in temperature on marine organisms is critical to predicting how they will cope with climate change. We evaluated the effects of OA as well as warming and cooling trend temperature on early reproductive traits of Lessonia trabeculata, a foundation kelp species. Sori discs were maintained for an exposure time (ET) of 3 (T3) and 7 (T7) days to one of two contrasting pCO2 levels (450 and 1100 μatm). In addition, at each pCO2 level, they were subjected to three temperature treatments: 15 °C (control), 10 °C (cool) and 19 °C (warm). Subsequently, we compared sorus photosynthetic performance (Fv/Fm), the number of meiospores released (MR) and their germination rate (GR) after 48 h of settlement, with values obtained from sori discs not exposed (DNE) to the treatments. The Fv/Fm measured for DNE was lower than at T3 and T7 at 10 and 15 °C but not at 19 °C. Regardless of temperature, we found no significant differences between MR measured at T0 and T3 were found. MR at T7 was significantly lower at 19 °C than at 10 and 15 °C. We found only aA significant reduction in MR in response to elevated pCO2 was only found at T3. The GR of meiospores released by DNE and then maintained for 48 h to 19 °C decreased significantly by ∼33 % when compared with those maintained for the same time at 10 and 15 °C. A similar, but more drastic reduction (∼54 %) in the GR was found in meiospores released by sori discs exposed for T3 and maintained for 48 h to 19 °C. We suggest that OA and warming trend will threaten the early establishment of this species with further consequences for the functioning of the associated ecosystem.

Graphical abstract

Under laboratory conditions were investigated the combined effect of pCO2, temperature and the exposure time on sorus photosynthetic performance (Fv/Fm) and meiospore performance (release and germination rate) of a habitat-forming kelp Lessonia trabeculata. The results suggest that important traits such as sorus photosynthetic performance, meiospores released and germination rate can be affected by those stressors and by the extent to which the sori are exposed. We concluded that ocean warming and ocean acidification might threaten the early establishment of this species with further consequences for the ecosystem functioning, goods and services in coastal environments.

Continue reading ‘Exposure time modulates the effects of climate change-related stressors on fertile sporophytes and early-life stage performance of a habitat-forming kelp species’

Rapid reduction of pH and CaCO3 saturation state in the Tsugaru Strait by the intensified Tsugaru warm current during 2012‐2019

Abstract

To examine the ocean acidification of coastal water as the result of the oceanic uptake of anthropogenic atmospheric CO2, we initiated acidification monitoring in the eastern part of the Tsugaru Strait, through which the Tsugaru Warm Current flows eastward from the Sea of Japan to the North Pacific. Annual mean pH and CaCO3 saturation state during 2012–2019 decreased considerably throughout all depths at rates of 0.0030−0.0051 yr−1 and 0.017−0.036 yr−1, respectively. These rates of decrease are faster than those caused by increasing atmospheric CO2, and faster than those observed during previous research. These fast rates are attributed to an enhanced increase in dissolved inorganic carbon concurrently with increases in salinity and density caused by elevated mixing of the upper and deeper waters from the Sea of Japan at the western part of the strait. The elevated mixing is attributable to the strengthening of the Tsugaru Warm Current.

Plain Language Summary

Approximately 30% of the total amount of CO2 released to the atmosphere by human activities has accumulated in the global ocean. This oceanic uptake of CO2 has resulted in ocean acidification. In coastal waters the acidification affects marine organisms, thus coastal ecosystems may be more vulnerable to acidification than the open ocean. To examine the extent to which acidification has advanced in the eastern part of the Tsugaru Strait, through which the Tsugaru Warm Current passes from the Sea of Japan to the North Pacific, we initiated a time‐series observation of acidification. The pH reduction is found to have enhanced considerably across the whole depth during 2012‐2019 at a rate faster than that caused by increasing atmospheric CO2 and at the highest rates observed during previous research. The rapid pH reduction is found to be attributable to the enhanced rate of increase of dissolved inorganic carbon concurrently with increases in salinity and density caused by elevated mixing of the upper and deeper waters from the Sea of Japan at the western strait due to the strengthening of the Tsugaru Warm Current. In other straits that are connected to the open ocean, the strengthening of their throughflow may also accelerate acidification.

Continue reading ‘Rapid reduction of pH and CaCO3 saturation state in the Tsugaru Strait by the intensified Tsugaru warm current during 2012‐2019’

CO2 capture by pumping surface acidity to the deep ocean

The majority of IPCC scenarios call for active CO2 removal (CDR) to remain below 2oC of warm- ing. On geological timescales, ocean uptake regulates atmospheric CO2 concentration, with two homeostats driving CO2 uptake: dissolution of deep ocean calcite deposits and terrestrial weathering of silicate rocks, acting on 1ka to 100ka timescales, respectively. Many current ocean-based CDR proposals effectively act to accelerate the latter. Here we present a method which relies purely on the redistribution and dilution of acidity from a thin layer of the surface ocean to a thicker layer of deep ocean, with the aim of reducing surface acidification and accelerating the former carbonate homeostasis. This downward transport could be seen analogous to the action of the natural biological carbon pump. The method offers advantages over other ocean CDR methods and direct air capture approaches (DAC): the conveyance of mass is minimized (acidity is pumped in situ to depth), and expensive mining, grinding and distribution of alkaline material is eliminated. No dilute substance needs to be concentrated, avoiding the Sherwood’s Rule costs typically encountered in DAC. Finally, no terrestrial material is added to the ocean, avoiding significant alteration of seawater ion concentrations or issues with heavy metal toxicity encountered in mineral-based alkalinity schemes. The artificial transport of acidity accelerates the natural deep ocean compensation by calcium carbonate. It has been estimated that the total compensation capacity of the ocean is on the order of 1500GtC. We show through simulation that pumping of ocean acidity could remove up to 150GtC from the atmosphere by 2100 with- out excessive increase of local ocean pH. For an acidity release below 2000m, the relaxation half-life of CO2 return to the atmosphere was found to be ∼2500 years (∼1000yr without account- ing for carbonate dissolution), with ∼85% retained for at least 300 years. The uptake efficiency and residence time were found to vary with the location of acidity pumping, and optimal areas were determined. Requiring only local resources (ocean water and energy), this method could be uniquely suited to utilize otherwise-unusable open ocean energy sources at scale. We examine technological pathways that could be used to implement it and present a brief techno-economic estimate of 130-250$/tCO2 at current prices and as low as 93$/tCO2 under modest learning-curve assumptions.

Continue reading ‘CO2 capture by pumping surface acidity to the deep ocean’

Differential responses in anti-predation traits of the native oyster Ostrea edulis and invasive Magallana gigas to ocean acidification and warming

Ocean acidification and warming (OAW) pose a threat to marine organisms, with particular negative effects on molluscs, and can jeopardize the provision of associated ecosystem services. As predation is an important factor shaping populations in the marine environment, the ability of organisms to retain traits valuable in predation resistance under OAW may be decisive for future population maintenance. We examine how exposure to seawater temperature (control: 16.8°C and warm: 20°C) and atmospheric pCO2 (ambient [~400], ~750, and ~1000 ppm) conditions affects traits linked to predation resistance (adductor muscle strength and shell strength) in two ecologically and economically important species of oysters (Magallana gigas and Ostrea edulis) and relate them to changes in morphometry and fitness (condition index, muscle and shell metrics). We show that O. edulis remained unimpacted following exposure to OAW scenarios. In contrast, the adductor muscle of M. gigas was 52% stronger under elevated temperature and ~750 ppm pCO2, and its shell was 44% weaker under combined elevated temperature and ~1000 ppm pCO2. This suggests greater resistance to mechanical predation toward the mid-21st century, but greater susceptibility toward the end of the century. For both species, individuals with more somatic tissue held an ecological advantage against predators; consequently, smaller oysters may be favoured by predators under OAW. By affecting fitness and predation resistance, OAW may be expected to induce shifts in predator-prey interactions and reshape assemblage structure due to species and size selection, which may consequently modify oyster reef functioning. This could in turn have implications for the provision of associated ecosystem services.

Continue reading ‘Differential responses in anti-predation traits of the native oyster Ostrea edulis and invasive Magallana gigas to ocean acidification and warming’

Large-scale interventions may delay decline of the Great Barrier Reef

On the iconic Great Barrier Reef (GBR), the cumulative impacts of tropical cyclones, marine heatwaves and regular outbreaks of coral-eating crown-of-thorns starfish (CoTS) have severely depleted coral cover. Climate change will further exacerbate this situation over the coming decades unless effective interventions are implemented. Evaluating the efficacy of alternative interventions in a complex system experiencing major cumulative impacts can only be achieved through a systems modelling approach. We have evaluated combinations of interventions using a coral reef meta-community model. The model consisted of a dynamic network of 3753 reefs supporting communities of corals and CoTS connected through ocean larval dispersal, and exposed to changing regimes of tropical cyclones, flood plumes, marine heatwaves and ocean acidification. Interventions included reducing flood plume impacts, expanding control of CoTS populations, stabilizing coral rubble, managing solar radiation and introducing heat-tolerant coral strains. Without intervention, all climate scenarios resulted in precipitous declines in GBR coral cover over the next 50 years. The most effective strategies in delaying decline were combinations that protected coral from both predation (CoTS control) and thermal stress (solar radiation management) deployed at large scale. Successful implementation could expand opportunities for climate action, natural adaptation and socioeconomic adjustment by at least one to two decades.

Continue reading ‘Large-scale interventions may delay decline of the Great Barrier Reef’

High-resolution carbonate system dynamics of Netarts Bay, OR from 2014 to 2019

Netarts Bay is a shallow, temperate, tidal lagoon located on the northern coast of Oregon and the site of the Whiskey Creek Shellfish Hatchery (WCSH). Data collected with an autonomous continuous flow-through system installed at WCSH capable of high-resolution (1 Hz) partial pressure of aqueous CO2 (pCO2) and hourly total dissolved inorganic carbon (TCO2) measurements, with combined measurement uncertainties of < 2.0% and 0.5%, respectively, is analyzed over the 2014–2019 interval. Summer upwelling, wintertime downwelling, and in situ bay biogeochemistry represent significant modes of the observed variability in carbonate system dynamics. Summer upwelling is associated with large amplitude diel pCO2 variability, elevated TCO2 and alkalinity, but weak variability in salinity. Wintertime downwelling is associated with bay freshening by both local and remote sources, a strong tidal signature in salinity, TCO2, and alkalinity, with diel pCO2 variability much less amplified when compared to summer. Further, analysis of alkalinity-salinity relationships suggests multiple water masses inhabiting the bay during 1 year: mixing of end-members associated with direct precipitation, coastal rivers, southward displacement of the Columbia River plume, California Current surface and deep upwelled waters. The importance of in-bay processes such as net community metabolism during intervals of high productivity are apparent. These direct measurements of pCO2 and TCO2 have been useful to local hatchery owners who have monitored intake waters following historic seed-production failures related to high-CO2 conditions exacerbated by ocean acidification.

Continue reading ‘High-resolution carbonate system dynamics of Netarts Bay, OR from 2014 to 2019’

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OA-ICC HIGHLIGHTS

Ocean acidification in the IPCC AR5 WG II

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