Ocean acidification increases the amount of dissolved inorganic carbon (DIC) available in seawater which can benefit photosynthesis in those algae that are currently carbon limited, leading to shifts in the structure and function of seaweed communities. Recent studies have shown that ocean acidification-driven shifts in seaweed community dominance will depend on interactions with other factors such as light and nutrients. The study of interactive effects of ocean acidification and warming can help elucidate the likely effects of climate change on marine primary producers. In this study, we investigated the ecophysiological responses of Cystoseira tamariscifolia (Hudson) Papenfuss. This large brown macroalga plays an important structural role in coastal Mediterranean communities. Algae were collected from both oligotrophic and ultraoligotrophic waters in southern Spain. They were then incubated in tanks at ambient (ca. 400–500 ppm) and high CO2 (ca. 1200–1300 ppm), and at 20 °C (ambient temperature) and 24 °C (ambient temperature +4 °C). Increased CO2 levels benefited the algae from both origins. Biomass increased in elevated CO2 treatments and was similar in algae from both origins. The maximal electron transport rate (ETRmax), used to estimate photosynthetic capacity, increased in ambient temperature/high CO2 treatments. The highest polyphenol content and antioxidant activity were observed in ambient temperature/high CO2 conditions in algae from both origins; phenol content was higher in algae from ultraoligotrophic waters (1.5–3.0%) than that from oligotrophic waters (1.0–2.2%). Our study shows that ongoing ocean acidification can be expected to increase algal productivity (ETRmax), boost antioxidant activity (EC50), and increase production of photoprotective phenols. Cystoseira tamariscifolia collected from oligotrophic and ultraoligotrophic waters were able to benefit from increases in DIC at ambient temperatures. Warming, not acidification, may be the key stressor for this habitat as CO2 levels continue to rise.
Posts Tagged 'Mediterranean'
Ecophysiological responses to elevated CO2 and temperature in Cystoseira tamariscifolia (Phaeophyceae)Published 20 March 2017 Science Leave a Comment
Tags: biological response, algae, physiology, photosynthesis, Mediterranean, laboratory, abundance, multiple factors, temperature, otherprocess
Tags: algae, biological response, corals, individualmodeling, laboratory, Mediterranean, modelling, molecular biology, multiple factors, photosynthesis, physiology, Red Sea, temperature
The anthropogenic increase in atmospheric CO2 that drives global warming and ocean acidification raises serious concerns regarding the future of corals, the main carbonate biomineralizers. Here we used transcriptome analysis to study the effect of long-term gradual temperature increase (annual rate), combined with lowered pH values, on a sub-tropical Red Sea coral, Stylophora pistillata, and on a temperate Mediterranean symbiotic coral Balanophyllia europaea. The gene expression profiles revealed a strong effect of both temperature increase and pH decrease implying for synergism response. The temperate coral, exposed to a twice as high range of seasonal temperature fluctuations than the Red Sea species, faced stress more effectively. The compensatory strategy for coping apparently involves deviating cellular resources into a massive up-regulation of genes in general, and specifically of genes involved in the generation of metabolic energy. Our results imply that sub-lethal, prolonged exposure to stress can stimulate evolutionary increase in stress resilience.
Experimental evidence of formation of transparent exopolymer particles (TEP) and POC export provoked by dust addition under current and high pCO2 conditionsPublished 23 February 2017 Science Leave a Comment
Tags: biogeochemistry, chemistry, laboratory, Mediterranean
The evolution of organic carbon export to the deep ocean, under anthropogenic forcing such as ocean warming and acidification, needs to be investigated in order to evaluate potential positive or negative feedbacks on atmospheric CO2 concentrations, and therefore on climate. As such, modifications of aggregation processes driven by transparent exopolymer particles (TEP) formation have the potential to affect carbon export. The objectives of this study were to experimentally assess the dynamics of organic matter, after the simulation of a Saharan dust deposition event, through the measurement over one week of TEP abundance and size, and to evaluate the effects of ocean acidification on TEP formation and carbon export following a dust deposition event. Three experiments were performed in the laboratory using 300 L tanks filled with filtered seawater collected in the Mediterranean Sea, during two ‘no bloom’ periods (spring at the start of the stratification period and autumn at the end of this stratification period) and during the winter bloom period. For each experiment, one of the two tanks was acidified to reach pH conditions slightly below values projected for 2100 (~ 7.6–7.8). In both tanks, a dust deposition event of 10 g m-2 was simulated at the surface. Our results suggest that Saharan dust deposition triggered the abiotic formation of TEP, leading to the formation of organic-mineral aggregates. The amount of particulate organic carbon (POC) exported was proportional to the flux of lithogenic particles to the sediment traps. Depending on the season, the POC flux following artificial dust deposition ranged between 38 and 90 mg m-2 over six experimental days. Such variability is likely linked to the seasonal differences in the quality and quantity of TEP-precursors initially present in seawater. Finally, these export fluxes were not significantly different at the completion of the three experiments between the two pH conditions.
Linking gene expression to productivity to unravel long- and short-term responses of seagrasses exposed to CO2 in volcanic ventsPublished 21 February 2017 Science Leave a Comment
Tags: biological response, field, Mediterranean, molecular biology, phanerogams, primary production, vents
Ocean acidification is a major threat for marine life but seagrasses are expected to benefit from high CO2. In situ (long-term) and transplanted (short-term) plant incubations of the seagrass Cymodocea nodosa were performed near and away the influence of volcanic CO2 vents at Vulcano Island to test the hypothesis of beneficial effects of CO2 on plant productivity. We relate, for the first time, the expression of photosynthetic, antioxidant and metal detoxification-related genes to net plant productivity (NPP). Results revealed a consistent pattern between gene expression and productivity indicating water origin as the main source of variability. However, the hypothesised beneficial effect of high CO2 around vents was not supported. We observed a consistent long- and short-term pattern of gene down-regulation and 2.5-fold NPP decrease in plants incubated in water from the vents and a generalized up-regulation and NPP increase in plants from the vent site incubated with water from the Reference site. Contrastingly, NPP of specimens experimentally exposed to a CO2 range significantly correlated with CO2 availability. The down-regulation of metal-related genes in C. nodosa leaves exposed to water from the venting site suggests that other factors than heavy metals, may be at play at Vulcano confounding the CO2 effects.
Adaptation policies and strategies as a response to ocean acidification and warming in the Mediterranean SeaPublished 16 February 2017 Science Leave a Comment
Tags: Mediterranean, mitigation, Policy, review
The ocean are a fundamental component of the Earth’s climate regulation, life and its carbon cycle. By burning fossil fuels since the Industrial Revolution, and thus emitting large amounts of carbon into the atmosphere, humans are changing the ocean in several ways. In particular, the ocean is absorbing atmospheric carbon dioxide (CO2) at such an unprecedented rate that it is rapidly changing its chemistry, resulting in “ocean acidification”, a reduction in pH, carbonate ion concentration and the ocean’s buffering capacity. Ocean acidification is a global environmental issue posing a threat to open ocean and coastal marine ecosystems, including semi-enclosed seas such as the Mediterranean Sea. (…)
Special edition of Estuarine, Coastal and Shelf Science – “Ocean acidification in the Mediterranean Sea: pelagic mesocosm experiments”Published 14 February 2017 Science Leave a Comment
Tags: biogeochemistry, biological response, BRcommunity, community composition, field, Mediterranean, mesocosms, multiple factors, nitrogen fixation, nutrients, otherprocess, physiology, phytoplankton, primary production, prokaryotes, virus, zooplankton
The topic of ocean acidification has received extensive attention in a recently published special edition of the journal Estuarine, Coastal and Shelf Science. Volume 186, Part A presents a series of 12 research papers focusing on pelagic mesocosm experiments conducted in the Mediterranean Sea in 2012 and 2013. Plankton plays a key role in the global carbon cycle. It is therefore important to project the evolution of plankton community structure and function in a future high-CO2 world. Several results from experiments conducted at the community level have shown increased rates of community primary production and shifts in community composition as a function of increasing pCO2. However, the great majority of these – experiments have been performed under high natural or nutrient-enriched conditions and very few data are available in areas with naturally low levels of nutrient and chlorophyll i.e. oligotrophic areas such as the Mediterranean Sea, although they represent a large and expanding part of the ocean surface. In the frame of the European Mediterranean Sea Acidification in a changing climate project (MedSeA; http://medsea-project.eu), large-scale in situ mesocosms (9 x 50 m3, 12 m deep) have been used to quantify the potential effects of CO2 enrichment in two coastal areas of the Mediterranean Sea: the bay of Calvi (Corsica, France) in June/July 2012 and the bay of Villefranche (France) in February/March 2013. These two experiments gathered the expertise of more than 25 scientists from 7 institutes and 6 countries (France, Greece, Spain, UK, Italy, Belgium, US).
Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents – insights from de novo transcriptomic analysisPublished 7 February 2017 Science Leave a Comment
Tags: algae, biological response, field, Mediterranean, molecular biology, physiology, primary production, vents
Ocean acidification is an emerging problem that is expected to impact ocean species to varying degrees. Currently, little is known about its effect on molecular mechanisms induced in fleshy macroalgae. To elucidate genome wide responses to acidification, a comparative transcriptome analysis was carried out between Sargassum vulgare populations growing under acidified conditions at volcanic CO2 vents and a control site. Several transcripts involved in a wide range of cellular and metabolic processes were differentially expressed. No drastic changes were observed in the carbon acquisition processes and RuBisCO level. Moreover, relatively few stress genes, including those for antioxidant enzymes and heat shock proteins, were affected. Instead, increased expression of transcripts involved in energy metabolism, photosynthetic processes, and ion homeostasis suggested that algae increased energy production to maintain ion-homeostasis and other cellular processes. Also, an increased allocation of carbon to cell wall and carbon storage was observed. A number of genes encoding proteins involved in cellular signaling, information storage and processing, and transposition were differentially expressed between the two conditions. The transcriptional changes of key enzymes were largely confirmed by enzymatic activity measurements. Altogether, the changes induced by acidification indicate an adaptation of growth and development of S. vulgare at the volcanic CO2 vents, suggesting that this fleshy alga exhibits a high plasticity to low pH and can adopt molecular strategies to grow also in future more acidified waters.