Posts Tagged 'respiration'

Coastal pH variability and the eco-physiological and behavioural response of a coastal fish species in light of future ocean acidification

Ocean acidification (OA) is a global phenomenon referring to a decrease in ocean pH and a perturbation of the seawater carbonate system due to ever-increasing atmospheric CO2 concentrations. In coastal environments, identifying the impacts of OA is complex due to the multiple contributors to pH variability by coastal processes, such as freshwater inflow, upwelling, hydrodynamic processes, and biological activity. The aim of this PhD study was to quantify the local processes occurring in a temperate coastal embayment, Algoa Bay in South Africa, that contribute to pH and carbonate chemistry variability over time (monthly and 24-hour) and space (~10 km) and examine how this variability impacts a local fish species, Diplodus capensis, also commonly known as ‘blacktail’. Algoa Bay, known for its complex oceanography, is an interesting location in which to quantify carbonate chemistry variability. To assess this variability, monitoring sites were selected to coincide with the Algoa Bay Sentinel Site long-term ecological research (LTER) and continuous monitoring (CMP) programmes. The average pH at offshore sites in the bay was 8.03 ± 0.07 and at inshore sites was 8.04 ± 0.15. High pH variability (~0.55–0.61 pH units) was recorded at both offshore (>10 m depth) and inshore sites (intertidal surf zones). Many sites in the bay, especially the atypical site at Cape Recife, exhibit higher than the average pH levels (>8.04), suggesting that pH variability may be biologically driven. This is further evidenced by high diurnal variability in pH (~0.55 pH units). Although the specific drivers of the high pH variability in Algoa Bay could not be identified, baseline carbonate chemistry conditions were identified, which is necessary information to design and interpret biological experiments. Long-term, continuous monitoring is required to improve understanding of the drivers of pH variability in understudied coastal regions, like Algoa Bay.

Continue reading ‘Coastal pH variability and the eco-physiological and behavioural response of a coastal fish species in light of future ocean acidification’

Seasonal photophysiological performance of adult western Baltic Fucus vesiculosus (Phaeophyceae) under ocean warming and acidification

Shallow coastal marine ecosystems are exposed to intensive warming events in the last decade, threatening keystone macroalgal species such as the bladder wrack (Fucus vesiculosus, Phaeophyceae) in the Baltic Sea. Herein, we experimentally tested in four consecutive benthic mesocosm experiments, if the single and combined impact of elevated seawater temperature (Δ + 5°C) and pCO2 (1100 ppm) under natural irradiance conditions seasonally affected the photophysiological performance (i.e., oxygen production, in vivo chlorophyll a fluorescence, energy dissipation pathways and chlorophyll concentration) of Baltic Sea Fucus. Photosynthesis was highest in spring/early summer when water temperature and solar irradiance increases naturally, and was lowest in winter (December to January/February). Temperature had a stronger effect than pCO2 on photosynthetic performance of Fucus in all seasons. In contrast to the expectation that warmer winter conditions might be beneficial, elevated temperature conditions and sub-optimal low winter light conditions decreased photophysiological performance of Fucus. In summer, western Baltic Sea Fucus already lives close to its upper thermal tolerance limit and future warming of the Baltic Sea during summer may probably become deleterious for this species. However, our results indicate that over most of the year a combination of future ocean warming and increased pCO2 will have slightly positive effects for Fucus photophysiological performance.

Continue reading ‘Seasonal photophysiological performance of adult western Baltic Fucus vesiculosus (Phaeophyceae) under ocean warming and acidification’

Cross‐generational response of a tropical sea urchin to global change and a selection event in a 43‐month mesocosm study

Long‐term experimental investigations of transgenerational plasticity (TGP) and transgenerational acclimatization to global change are sparse in marine invertebrates. Here, we test the effect of ocean warming and acidification over a 25‐month period of Echinometra sp. A sea urchins whose parents were acclimatized at ambient or one of two near‐future (projected mid‐ and end‐ of the 21st century) climate scenarios for 18 months. Several parameters linked to performance exhibited strong effects of future ocean conditions at 9 months of age. The Ambient‐Ambient group (A‐A, both F0 and F1 at ambient conditions) was significantly larger (21%) and faster in righting response (31%) compared to other groups. A second set of contrasts revealed near‐future scenarios caused significant negative parental carryover effects. Respiration at 9 months was depressed by 59% when parents were from near‐future climate conditions, and righting response was slowed by 28%. At ten months, a selective pathogenic mortality event lead to significantly higher survival rates of A‐A urchins. Differences in size and respiration measured prior to the mortality were absent after the event, while a negative parental effect on righting (29% reduction) remained. The capacity to spawn at the end of the experiment was higher in individuals with ambient parents (50%) compared to other groups (21%) suggesting persistent parental effects. Obtaining different results at different points in time illustrates the importance of longer‐term and multi‐generation studies to investigate effects of climate change. Given some animals in all groups survived the pathogenic event and that effects on physiology (but not behavior) among groups were eliminated after the mortality, we suggest that similar events could constitute selective sweeps, allowing genetic adaptation. However, given the observed negative parental effects and reduced potential for population replenishment it remains to be determined if selection would be sufficiently rapid to rescue this species from climate change effects.

Continue reading ‘Cross‐generational response of a tropical sea urchin to global change and a selection event in a 43‐month mesocosm study’

Parental whole life cycle exposure modulates progeny responses to ocean acidification in slipper limpets

Multigenerational exposure is needed to assess the evolutionary potential of organisms in the rapidly changing seascape. Here, we investigate if there is a transgenerational effect of ocean acidification exposure on a calyptraeid gastropod such that long‐term exposure elevates offspring resilience. Larvae from wild type Crepidula onyx adults were reared from hatching until sexual maturity for over 36 months under three pH conditions (pH 7.3, 7.7, and 8.0). While the survivorship, growth, and respiration rate of F1 larvae were unaffected by acute ocean acidification (OA), long‐term and whole life‐cycle exposure significantly compromised adult survivorship, growth, and reproductive output of the slipper limpets. When kept under low pH throughout their life cycle, only 6% of the F1 slipper limpets survived pH 7.3 conditions after ~2.5 years and the number of larvae they released was ~10% of those released by the control. However, the F2 progeny from adults kept under the long‐term low pH condition hatched at a comparable size to those in medium and control pH conditions. More importantly, these F2 progeny from low pH adults outperformed F2 slipper limpets from control conditions; they had higher larval survivorship and growth, and reduced respiration rate across pH conditions, even at the extreme low pH of 7.0. The intragenerational negative consequences of OA during long‐term acclimation highlights potential carryover effects and ontogenetic shifts in stress vulnerability, especially prior to and during reproduction. Yet, the presence of a transgenerational effect implies that this slipper limpet, which has been widely introduced along the West Pacific coasts, has the potential to adapt to rapid acidification.

Continue reading ‘Parental whole life cycle exposure modulates progeny responses to ocean acidification in slipper limpets’

Bacterial communities are more sensitive to ocean acidification than fungal communities in estuarine sediments

Ocean acidification (OA) in estuaries is becoming a global concern, and may affect microbial characteristics in estuarine sediments. Bacterial communities in response to acidification in this habitat have been well discussed; however, knowledge about how fungal communities respond to OA remains poorly understood. Here, we explored the effects of acidification on bacterial and fungal activities, structures and functions in estuarine sediments during a 50-day incubation experiment. Under acidified conditions, activities of three extracellular enzymes related to nutrient cycling were inhibited and basal respiration rates were decreased. Acidification significantly altered bacterial communities and their interactions, while weak alkalization had a minor impact on fungal communities. We distinguished pH-sensitive/tolerant bacteria and fungi in estuarine sediments, and found that only pH-sensitive/tolerant bacteria had strong correlations with sediment basal respiration activity. FUNGuild analysis indicated that animal pathogen abundances in sediment were greatly increased by acidification, while plant pathogens were unaffected. High-throughput quantitative PCR-based SmartChip analysis suggested that the nutrient cycling-related multifunctionality of sediments was reduced under acidified conditions. Most functional genes associated with nutrient cycling were identified in bacterial communities and their relative abundances were decreased by acidification. These new findings highlight that acidification in estuarine regions affects bacterial and fungal communities differently, increases potential pathogens and disrupts bacteria-mediated nutrient cycling.

Continue reading ‘Bacterial communities are more sensitive to ocean acidification than fungal communities in estuarine sediments’

Seasonal marine carbon system processes in an Arctic coastal landfast sea ice environment observed with an innovative underwater sensor platform

Studying carbon dioxide in the ocean helps to understand how the ocean will be impacted by climate change and respond to increasing fossil fuel emissions. The marine carbonate system is not well characterized in the Arctic, where challenging logistics and extreme conditions limit observations of atmospheric CO2 flux and ocean acidification. Here, we present a high-resolution marine carbon system data set covering the complete cycle of sea-ice growth and melt in an Arctic estuary (Nunavut, Canada). This data set was collected through three consecutive yearlong deployments of sensors for pH and partial pressure of CO2 in seawater (pCO2sw) on a cabled underwater observatory. The sensors were remarkably stable compared to discrete samples: While corrections for offsets were required in some instances, we did not observe significant drift over the deployment periods. Our observations revealed a strong seasonality in this marine carbon system. Prior to sea-ice formation, air–sea gas exchange and respiration were the dominant processes, leading to increasing pCO2sw and reduced aragonite saturation state (ΩAr). During sea-ice growth, water column respiration and brine rejection (possibly enriched in dissolved inorganic carbon, relative to alkalinity, due to ikaite precipitation in sea ice) drove pCO2sw to supersaturation and lowered ΩAr to < 1. Shortly after polar sunrise, the ecosystem became net autotrophic, returning pCO2sw to undersaturation. The biological community responsible for this early switch to autotrophy (well before ice algae or phytoplankton blooms) requires further investigation. After sea-ice melt initiated, an under-ice phytoplankton bloom strongly reduced aqueous carbon (chlorophyll-a max of 2.4 µg L–1), returning ΩAr to > 1 after 4.5 months of undersaturation. Based on simple extrapolations of anthropogenic carbon inventories, we suspect that this seasonal undersaturation would not have occurred naturally. At ice breakup, the sensor platform recorded low pCO2sw (230 µatm), suggesting a strong CO2 sink during the open water season.

Continue reading ‘Seasonal marine carbon system processes in an Arctic coastal landfast sea ice environment observed with an innovative underwater sensor platform’

Risks to the stability of coral reefs in the South China Sea: an integrated biomarker approach to assess the physiological responses of Trochus niloticus to ocean acidification and warming

Highlights

  • OA and OW have deleterious effects on the fitness of T. niloticus.
  • Co-exposure of OA and OW is the most stressful condition.
  • OA and OW may adversely affect population replenishment of T. niloticus.

Abstract

Scientific researches have clearly indicated that ocean acidification and warming poses serious threats to coral reef ecosystems. In coral reef ecosystems, herbivorous gastropods have an important function in maintaining the stability of the ecosystem due to controlling the abundance and growth of macroalgal, which compete for nutrients and space with coral. However, limited knowledge is available on the physiological responses of the specific keystone species to the increased ocean acidity and thermal stress. In this study, we evaluated the effects of ocean acidification (OA) and warming (OW) on an herbivorous gastropod Trochus niloticus commonly found on intertidal and shallow subtidal coral reefs in the South China Sea, on the aspect of immune responses (total hemocyte counts, reactive oxygen species level and apoptosis rate), oxidative stress (lipid peroxidation level, antioxidant enzyme activities), neurotoxicity (acetylcholinesterase activity), and energy metabolism (respiration rate and cellular energy allocation), after a 28-days exposure experiment to acidic (pH 7.6) and/or thermal (30 °C) seawater. Our results demonstrated that both OA and OW could lead to physiological disturbances of the herbivorous top-shells, including impaired immune functions and oxidative balance, neurotoxicity, and disorder of energy metabolism. Furthermore, results of integrated biomarker response (IBR) confirmed that the overall fitness of T. niloticus were deleteriously impacted by OA and OW, and were more stressed under the co-exposure condition. These results indicated that increased acidity and temperature in the future ocean might impair the viability of T. niloticus in the long-run, which will indulge the proliferation of macroalgae and lead to degradation of the coral reef ecosystem.

Continue reading ‘Risks to the stability of coral reefs in the South China Sea: an integrated biomarker approach to assess the physiological responses of Trochus niloticus to ocean acidification and warming’

Partner preference in the intertidal: possible benefits of ocean acidification to sea anemone-algal symbiosis

Ocean acidification (OA) threatens many marine species and is projected to become more severe over the next 50 years. Areas of the Salish Sea and Puget Sound that experience seasonal upwelling of low pH water are particularly susceptible to even lower pH conditions. While ocean acidification literature often describes negative impacts to calcifying organisms, including economically important shellfish, and zooplankton, not all marine species appear to be
threatened by OA. Photosynthesizing organisms, in particular, may benefit from increased levels of CO2. The aggregating anemone (Anthopleura elegantissima), a common intertidal organism throughout the northeast Pacific, hosts two photosynthetic symbionts: Symbiodinium muscatinei (a dinoflagellate) and Elliptochloris marina (a chlorophyte). The holobiont, therefore, consists of both a cnidarian host and a photosymbiont that could be affected differently by the changing levels of environmental CO2. To determine the effects of OA on this important marine organism, A. elegantissima in each of four symbiotic conditions (hosting S. muscatinei, hosting E. marina, hosting mixed symbiont assemblages, or symbiont free) were subjected to one of three pCO2 levels (800 ppm, 1200 ppm, or 1800 ppm) of OA for 10 weeks. At regular intervals, gross photosynthesis and density of the symbionts, respiration rate of the hosts, levels of reactive oxygen species (ROS) in the host, and percent of organic carbon received by the host from the symbiont (CZAR) were measured. Over the 10-week period of the experiment, the densities of symbionts responded differently to an increase in pCO2, increasing in anemones hosting S. muscatinei but decreasing for those hosting E. marina. Similarly, anemones of mixed symbiont complement that started with approximately 50% of each symbiont type shifted toward a higher percentage of S. muscatinei with higher pCO2. Both gross photosynthesis and dark respiration were significantly affected by pCO2 and symbiont state, though we cannot say that the symbiontsv responded differently to increased OA. Symbiont state was a significant predictor for ROS concentration, with greatest levels seen in anemones hosting E. marina and for CZAR score, with greatest levels in anemones hosting S. muscatinei, our linear models did not reveal pCO2 as a significant factor in these responses. Together, these results suggest that S. muscatinei may benefit from elevated pCO2 levels and that A. elegantissima hosting that symbiont may have a competitive advantage under some future scenarios of ocean acidification.

Continue reading ‘Partner preference in the intertidal: possible benefits of ocean acidification to sea anemone-algal symbiosis’

Ecosystem composition and environmental factors as drivers of pH on barrier reefs

Tropical coral reefs are both biologically diverse and economically important ecosystems, yet are under threat globally, facing a multitude of stressors including global warming, ocean acidification, nutrient loading, over-fishing and sedimentation. Reef building corals precipitate an aragonite skeleton (CaCO3), which forms the base of the coral reef ecosystem, but it is this skeleton, which makes them sensitive to changes in ocean pH. To precipitate their skeletons, corals raise their internal pH, as seawater pH decreases this increases the energy demands needed to facilitate calcification. Furthermore, reductions in coral calcification has significant implications for reef health, potentially altering community structure with reef-wide consequences. Global ocean pH is decreasing due to rising atmospheric concentrations of CO2, however, dynamic ecosystems, alongside carbon and freshwater input from land, may result in coastal ocean pH being lower than is predicted by open ocean models. While it is predicted than ocean pH will decrease by 0.3 units by 2100 if emissions are not curbed, coral reefs, particularly those near major river outflow, may already be experiencing pH values similar to that of future scenarios.

Our aim was to determine the factors which influence pH in coastal reef systems and thus potentially mitigate or exacerbate atmospheric CO2 mediated ocean acidification. This was achieved by contrasting reefs in distinct environmental settings and collecting data over a sufficient temporal resolution to permit the identification of pertinent drivers. To accomplish this we deployed fixed point observatories in the distinct reefs of Belize (fore and back reef sites), Fiji and Dominica. These custom-built platforms were equipped with a spectrophotometric pH sensor and a conductivity, temperature and dissolved oxygen (CT-DO) sensor from which data was logged at 30-120 minute intervals.

A strong diel cycle in pH, O2 and temperature was observed at all reef sites in response to the changing balance of respiration and photosynthesis. However, the range of these changes varied between the different sites – Belize fore reef (pH 7.849­ – 8.000), Belize back reef (pH 7.897 – 8.039), Fiji (pH 7.951 – 8.0950) and Dominica (pH 7.843 – 8.144). Meteorological conditions, such as wind direction, affected the amplitude of diurnal pH variability and its relationship with other parameters, likely by influencing mixing and the spatial distribution of seawater and freshwater endmembers. The relationship between pH and O2 varied between sites reflecting differences in ecosystem processes (e.g. calcification and primary production) and ecosystem composition (e.g. hard coral and algae cover, proximity to seagrass). Our data confirms that different reef sites are subject to varying degrees of ocean acidification and that controls on pH vary between environments. Furthermore, it highlights the need for widespread high-resolution monitoring to identify, and where possible enact protective measures, in vulnerable reef regions. As coral reefs continue to experience ocean acidification our data also serves to document baseline conditions against which future changes can be assessed.

Continue reading ‘Ecosystem composition and environmental factors as drivers of pH on barrier reefs’

Unexpected role of communities colonizing dead coral substrate in the calcification of coral reefs

Global and local anthropogenic stressors such as climate change, acidification, overfishing, and pollution are expected to shift the benthic community composition of coral reefs from dominance by calcifying organisms to dominance by non‐calcifying algae. These changes could reduce the ability of coral reef ecosystems to maintain positive net calcium carbonate accretion. However, relationships between community composition and calcification rates remain unclear. We performed field experiments to quantify the metabolic rates of the two most dominant coral reef substrate types, live coral and dead coral substrate colonized by a mixed algal assemblage, using a novel underwater respirometer. Our results revealed that calcification rates in the daytime were similar for the live coral and dead coral substrate communities. However, in the dark, while live corals continued to calcify at slower rates, the dead coral substrate communities exhibited carbonate dissolution. Daytime net photosynthesis of the dead coral substrate communities was up to five times as much as for live corals, which we hypothesize may have created favorable conditions for the precipitation of carbonate minerals. We conclude that: (1) calcification from dead coral substrate communities can contribute to coral reef community calcification during the day, and (2) dead coral substrate communities can also contribute to carbonate mineral dissolution at night, decreasing ecosystem calcification over a diel cycle. This provides evidence that reefs could shift from slow, long‐term accretion of calcium carbonate to a state where large daily cycling of calcium carbonate occurs, but with little or no long‐term accumulation of the carbonate minerals needed to sustain the reef against erosional forces.

Continue reading ‘Unexpected role of communities colonizing dead coral substrate in the calcification of coral reefs’

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Ocean acidification in the IPCC AR5 WG II

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