Expansion of land-based systems in fish farms elevate the content of metabolic carbon dioxide (CO2) in the water. High CO2 is suggested to increase the bone mineral content in Atlantic salmon (Salmo salar, L.). Conversely, low dietary phosphorus (P) halts bone mineralization. This study examines if high CO2 can counteract reduced bone mineralization imposed by low dietary P intake. Atlantic salmon post-seawater transfer (initial weight 207.03 g) were fed diets containing 6.3 g/kg (0.5P), 9.0 g/kg (1P), or 26.8 g/kg (3P) total P for 13 weeks. Atlantic salmon from all dietary P groups were reared in seawater which was not injected with CO2 and contained a regular CO2 level (5 mg/L) or in seawater with injected CO2 thus raising the level to 20 mg/L. Atlantic salmon were analyzed for blood chemistry, bone mineral content, vertebral centra deformities, mechanical properties, bone matrix alterations, expression of bone mineralization, and P metabolism-related genes. High CO2 and high P reduced Atlantic salmon growth and feed intake. High CO2 increased bone mineralization when dietary P was low. Atlantic salmon fed with a low P diet downregulated the fgf23 expression in bone cells indicating an increased renal phosphate reabsorption. The current results suggest that reduced dietary P could be sufficient to maintain bone mineralization under conditions of elevated CO2. This opens up a possibility for lowering the dietary P content under certain farming conditions.
Continue reading ‘Elevated water CO2 can prevent dietary-induced osteomalacia in post-smolt Atlantic salmon (Salmo salar, L.)’Posts Tagged 'fish'
Elevated water CO2 can prevent dietary-induced osteomalacia in post-smolt Atlantic salmon (Salmo salar, L.)
Published 24 May 2023 Science Leave a CommentTags: biological response, fish, growth, laboratory, molecular biology, morphology, North Atlantic, performance, physiology
Recalibrating the significance of the decline effect in fish ocean acidification research
Published 16 May 2023 Science ClosedTags: biological response, fish, performance, review
The recently described decline effect in ocean acidification impacts on fish behaviour should not be equated with negligible effects. Here, existing mechanistic data are used to argue for continued research and cautions against “throwing the baby out with the bathwater.”
The past few years have seen a seismic shift in the scientific consensus of ocean acidification and its impacts on fishes, particularly the adverse effects on behaviour. Foundational early work on coral reef fishes detailed olfactory disturbances that left fish unable to detect or discriminate predator cues and necessary habitat settlement cues—both of which were held up as potentially serious consequences of ocean acidification that may threaten global fish populations [1]. A decade later, Clark and colleagues published a rigorous follow-up that questioned the reproducibility of the early work on fish behaviour [2], and while several design aspects were disputed [3], the prevailing opinions on the behavioural effects of ocean acidification on fishes began to change. The recently published meta-analysis by Clements and colleagues [4] reinforced this shift by demonstrating a decline in effect size response ratios over time in studies exploring the impacts of ocean acidification on fish behaviour. The authors argued that ocean acidification has negligible effects on fish behaviour. More alarming was the determination that a University investigative panel concluded that a prominent author of the early ocean acidification studies committed scientific misconduct in the form of data fabrication and falsification [5]. This has led to one retraction of a high-impact work on coral reef fishes, although as of this writing, no ocean acidification papers have been retracted nor any expressions of concern been issued. Nonetheless, guilt by association has coloured the field of ocean acidification and fish behaviour. Despite all of this, I would urge the scientific community to remember the classic idiom and not “throw the baby out with the bathwater”.
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Continue reading ‘Recalibrating the significance of the decline effect in fish ocean acidification research’The effects of the “deadly trio” (warming, acidification, and deoxygenation) on fish early ontogeny
Published 11 May 2023 Science ClosedTags: biological response, fish, laboratory, morphology, mortality, multiple factors, North Atlantic, oxygen, performance, physiology, reproduction, temperature
The interaction between increased dissolved carbon dioxide, rising temperatures, and oxygen loss – the so-called “deadly trio” – is expected to strongly affect marine biota over the coming years, potentially undermining ocean services and uses. Nonetheless, no study has so far scrutinized the cumulative impact of these three stressors on fish embryonic and larval stages, known to be particularly vulnerable to environmental stress. To fill this knowledge gap, we implemented a fully multi-factorial design to investigate the effects of acute warming (Δ + 4°C; 22 ºC), acidification (Δ − 0.4 pH units; ~ 7.7 pCO2) and deoxygenation (Δ − 60% O2 saturation, ~ 3 mg O2 l− 1) over a comprehensive array of physiological (hatching success, survival rates, deformities rates, and heart rates) and behavioural responses (larvae responsiveness and phototaxis) across the early ontogeny of the temperate gilthead seabream (Sparus aurata). Deoxygenation was the main driver of negative impacts in the hatching success (64.25%), survival (46.71%), and heart rates (31.99%) of recently hatched larvae, being generally further exacerbated when warming and acidification co-occurred. On the other hand, acidification was the only factor to induce a significant decrease in the proportion of phototactic behaviour (50%). The behavioural and physiological responses showed to be highly correlated across experimental treatments, specifically, phototaxis was negatively correlated with the incidence of malformations, and positively correlated with heart rates. Overall, our findings indicate that the interaction between warming, acidification, and deoxygenation is markedly detrimental to fish early developmental stages, impacting several key features at this critical life stage that may eventually cause adverse carry-over effects. Importantly, our analysis highlights the need to assess the concurrent impacts of stressors’ interaction on marine taxa to better predict future ecosystem responses to ocean changes.
Continue reading ‘The effects of the “deadly trio” (warming, acidification, and deoxygenation) on fish early ontogeny’Coral persistence despite marginal conditions in the Port of Miami
Published 4 May 2023 Science ClosedTags: abundance, algae, biological response, BRcommunity, chemistry, community composition, corals, field, fish, North Pacific, otherprocess
Coral cover has declined worldwide due to anthropogenic stressors that manifest on both global and local scales. Coral communities that exist in extreme conditions can provide information on how these stressors influence ecosystem structure, with implications for their persistence under future conditions. The Port of Miami is located within an urbanized environment, with active coastal development, as well as commercial shipping and recreational boating activity. Monitoring of sites throughout the Port since 2018 has revealed periodic extremes in temperature, seawater pH, and salinity, far in excess of what have been measured in most coral reef environments. Despite conditions that would kill many reef species, we have documented diverse coral communities growing on artificial substrates at these sites—reflecting remarkable tolerance to environmental stressors. Furthermore, many of the more prevalent species within these communities are now conspicuously absent or in low abundance on nearby reefs, owing to their susceptibility and exposure to stony coral tissue loss disease. Natural reef frameworks, however, are largely absent at the urban sites and while diverse fish communities are documented, it is unlikely that these communities provide the same goods and services as natural reef habitats. Regardless, the existence of these communities indicates unlikely persistence and highlights the potential for coexistence of threatened species in anthropogenic environments, provided that suitable stewardship strategies are in place.
Continue reading ‘Coral persistence despite marginal conditions in the Port of Miami’Acidification scenario of Cox’s Bazar coast of the Bay of Bengal, Bangladesh and its influence on fish larvae abundance
Published 3 May 2023 Science ClosedTags: abundance, biological response, chemistry, community composition, field, fish, Indian, otherprocess
Ocean acidification is caused mainly by atmospheric carbon dioxide stored in the ocean. Ocean acidification is considered a major threat to aquatic life, and how it influences the abundance of marine fish larvae is still unclear. This research was designed to measure the current ocean acidification scenario of the Cox’s Bazar coast of the Bay of Bengal, Bangladesh, and its probable influence on the abundance of fish larvae. Three research stations were selected: Bakkhali river estuary, Naf river estuary, and Rezu Khal. Monthly sampling was done, and larvae sample was collected from the surface water column (depth: 0.5 m) using a bongo net. Water parameters such as temperature, salinity, total alkalinity, and pH were determined using laboratory protocol. The seacarb package of the R programming language was used to determine ocean acidification factors. The Bakkhali river estuary showed the highest partial carbon dioxide (143.99 ± 102.27 μatm) and the lowest pH (8.27 ± 0.21). A total of 19 larvae families were identified, and the highest larval count was found in Rezu Khal (390 larvae/1000 m3), while the lowest was found in the Bakkhali river (3 larvae/1000 m3). Clupeidae, Myctophidae, and Engraulidae comprised more than 50% of the identified larvae. Blenniidae, Carangidae, Clupeidae, Engraulidae, and Gobiidae were found in all three seasons. Most of the larvae families showed the highest mean abundance under less pCO2. A negative correlation was observed between larvae and acidification factors such as pCO2, HCO3−, and dissolved inorganic carbon (DIC). The study revealed that acidification parameters of the Cox’s Bazar coast were not in an acute state for the aquatic organisms’ survival, but fish larvae abundance could be declined with raises in the partial carbon dioxide. The results of this study may aid in developing a management plan for conserving Bangladesh’s marine and coastal fish.
Continue reading ‘Acidification scenario of Cox’s Bazar coast of the Bay of Bengal, Bangladesh and its influence on fish larvae abundance’Future shock: ocean acidification and seasonal water temperatures alter the physiology of competing temperate and coral reef fishes
Published 28 April 2023 Science ClosedTags: biological response, fish, laboratory, multiple factors, physiology, South Pacific, temperature
Highlights
- Temperate and coral reef fishes were exposed to ocean acidification and ocean warming
- Coral reef fish decreased physiological performance in future winters (20 °C + OA)
- Coral reef fish increased lipid energy storage in future winter conditions
- Temperate fish increased oxidative damage in future summers (26 °C + OA)
- Future climate can modify the physiology of temperate and coral fishes seasonally
Abstract
Climate change can directly (physiology) and indirectly (novel species interactions) modify species responses to novel environmental conditions during the initial stages of range shifts. Whilst the effects of climate warming on tropical species at their cold-water leading ranges are well-established, it remains unclear how future seasonal temperature changes, ocean acidification, and novel species interactions will alter the physiology of range-shifting tropical and competing temperate fish in recipient ecosystems. Here we used a laboratory experiment to examine how ocean acidification, future summer vs winter temperatures, and novel species interactions could affect the physiology of competing temperate and range-extending coral reef fish to determine potential range extension outcomes. In future winters (20 °C + ocean acidification) coral reef fish at their cold-water leading edges showed reduced physiological performance (lower body condition and cellular defence, and higher oxidative damage) compared to present-day summer (23 °C + control pCO2) and future summer conditions (26 °C + ocean acidification). However, they showed a compensatory effect in future winters through increased long-term energy storage. Contrastingly, co-shoaling temperate fish showed higher oxidative damage, and reduced short-term energy storage and cellular defence in future summer than in future winter conditions at their warm-trailing edges. However, temperate fish benefitted from novel shoaling interactions and showed higher body condition and short-term energy storage when shoaling with coral reef fish compared to same-species shoaling. We conclude that whilst during future summers, ocean warming will likely benefit coral reef fishes extending their ranges, future winter conditions may still reduce coral reef fish physiological functioning, and may therefore slow their establishment at higher latitudes. In contrast, temperate fish species benefit from co-shoaling with smaller-sized tropical fishes, but this benefit may dissipate due to their reduced physiological functioning under future summer temperatures and increasing body sizes of co-shoaling tropical species.
Continue reading ‘Future shock: ocean acidification and seasonal water temperatures alter the physiology of competing temperate and coral reef fishes’Effects of dissolved carbon dioxide on growth and vertebral column of hybrid marine grouper (Epinephelus fuscoguttatus × E. lanceolatus) early advanced larvae
Published 24 April 2023 Science ClosedTags: biological response, fish, growth, laboratory, morphology, mortality, performance, physiology, South Pacific
Highlights
- Ocean acidification negatively impacted the early advanced larvae of the marine hybrid tiger grouper × giant grouper (TG × GG).
- Worst growth, survival, weight, food consumption, and conversion rates at 1000 ppm CO2.
- Deformed vertebral columns were observed at 1000 ppm CO2, while normal vertebral column observed at 400 ppm CO2.
- This study provides guidelines for future studies on TG × GG larvae or other marine fish larvae under elevated CO2 concentrations.
Abstract
This study investigated the effects of different dissolved carbon dioxide (CO2) concentrations (400, 700, and 1000 ppm) on the growth and vertebral column formation of hybrid tiger grouper × giant grouper (TG × GG) in their advanced larval stage under controlled laboratory conditions for 12 weeks. Growth parameters, including specific growth rate (SGR), survival rate, food consumption (FC), and food conversion rate (FCR), were calculated at the end of the experiment. Vertebral column formation was analysed using X-radiography and osteology methods. The results showed that all growth parameters were significantly affected by CO2 concentration, with the best performances observed under 400 ppm CO2. The highest statistically significant (p < 0.05) SGR, survival rate, and FC were observed under 400 ppm CO2, whereas the lowest was observed under 1000 ppm CO2. The lowest FCR (0.40, p < 0.05) was observed in 400 ppm CO2 and the highest was observed at 1000 ppm CO2 (0.59, p < 0.05). Furthermore, larvae without vertebral column malformations were observed in 400 ppm CO2, while larvae with small angles of kyphosis were observed in 700 ppm CO2, and larvae with kyphosis, lordosis, and vertebral compression were observed in 1000 ppm CO2. Only six spine measurements out of 31 obtained under different CO2 concentrations were significantly different (p < 0.05). Overall, the results suggest that CO2 concentration plays a crucial role in the growth and vertebral column formation of TG × GG in their advanced larval stage. The optimal CO2 concentration for the aquaculture of TG × GG in their advanced larval stage was found to be 400 ppm or lower. This study highlights the importance of maintaining optimal CO2 concentrations to enhance the growth and health of fish in aquaculture systems…
Continue reading ‘Effects of dissolved carbon dioxide on growth and vertebral column of hybrid marine grouper (Epinephelus fuscoguttatus × E. lanceolatus) early advanced larvae’Combined effects of climate change and BDE-209 dietary exposure on the behavioural response of the white seabream, Diplodus sargus
Published 14 April 2023 Science ClosedTags: biological response, fish, laboratory, multiple factors, performance, temperature, toxicants
Highlights
- Fish were exposed to acidification, warming and BDE-209 via diet for 56 days.
- BDE-209 lowered fish awareness of a risky situation and increased fish activity.
- Interaction of BDE-209 with acidification and/or warming altered fish responses.
- Acidification plus BDE-209 exposure increased fish anxiety and shoal cohesion.
- Warming plus BDE-209 exposure increased anxiety and reversed fish lateralization.
Abstract
Decabromodiphenyl-ether (BDE-209) is a persistent organic pollutant ubiquitously found in marine environments worldwide. Even though this emerging chemical contaminant is described as highly toxic, bioaccumulative and biomagnifiable, limited studies have addressed the ecotoxicological implications associated with its exposure in non-target marine organisms, particularly from a behavioural standpoint. Alongside, seawater acidification and warming have been intensifying their impacts on marine ecosystems over the years, compromising species welfare and survival. BDE-209 exposure as well as seawater acidification and warming are known to affect fish behaviour, but information regarding their interactive effects is not available. In this study, long-term effects of BDE-209 contamination, seawater acidification and warming were studied on different behavioural traits of Diplodus sargus juveniles. Our results showed that D. sargus exhibited a marked sensitivity in all the behaviour responses after dietary exposure to BDE-209. Fish exposed to BDE-209 alone revealed lower awareness of a risky situation, increased activity, less time spent within the shoal, and reversed lateralization when compared to fish from the Control treatment. However, when acidification and/or warming were added to the equation, behavioural patterns were overall altered. Fish exposed to acidification alone exhibited increased anxiety, being less active, spending more time within the shoal, while presenting a reversed lateralization. Finally, fish exposed to warming alone were more anxious and spent more time within the shoal compared to those of the Control treatment. These novel findings not only confirm the neurotoxicological attributes of brominated flame retardants (like BDE-209), but also highlight the relevance of accounting for the effects of abiotic variables (e.g. pH and seawater temperature) when investigating the impacts of environmental contaminants on marine life.
Continue reading ‘Combined effects of climate change and BDE-209 dietary exposure on the behavioural response of the white seabream, Diplodus sargus’Marked recent declines in boron in Baltic Sea cod otoliths – a bellwether of incipient acidification in a vast hypoxic system?
Published 11 April 2023 Science ClosedTags: Baltic, biological response, chemistry, field, fish, multiple factors, oxygen, physiology, salinity
Ocean acidification is spreading globally as a result of anthropogenic CO2 emissions, but the Baltic Sea has until recently been thought to be relatively well-buffered by terrigenous inputs of alkalinity from its watershed. We discovered a 3- to 5-fold decline in boron (as B : Ca) in otoliths of eastern Baltic Sea cod (EBC) between the late 1990s and 2021. B : Ca is positively proportional to pH in carbonates, as B in the form of borate is taken up in the CaCO3 matrix. Examining a time series of EBC otoliths, we found varying levels of B : Ca since the 1980s, with the most recent years at an all-time low during this period. This trend correlates with declines in pH and dissolved oxygen, but not with changes in salinity. We examined possible physiological influences on B : Ca by including a collection of healthy Icelandic cod as an out-group. Icelandic cod otoliths showed strongly positive correlations of B : Ca with physiologically regulated P : Ca; this was not the case for EBC. Finally, B : Ca in EBC otoliths is anti-correlated to some extent with Mn : Mg, a proposed proxy for hypoxia exposure. This negative relationship is hypothesized to reflect the dual phenomena of hypoxia and acidification as a result of decomposition of large algal blooms. Taken together, the otolith biomarkers Mn : Mg and B : Ca suggest a general increase in both hypoxia and acidification within the Baltic intermediate and deep waters in the last decade reflected in cod otoliths.
Continue reading ‘Marked recent declines in boron in Baltic Sea cod otoliths – a bellwether of incipient acidification in a vast hypoxic system?’Review of warming and acidification effects to the ecotoxicity of pharmaceuticals on aquatic organisms in the era of climate change
Published 6 April 2023 Science ClosedTags: biological response, crustaceans, fish, metals, molecular biology, mollusks, mortality, multiple factors, physiology, respiration, review, temperature, toxicants
Highlights
- Acidification and warming modulates the ecotoxicity of pharmaceuticals.
- Biochemical, cellular and behavioral biomarkers show a response.
- Trends of change in acute and chronic toxicity were drug dependent.
- Acidification modified the toxicity of selected ionizable pharmaceuticals.
- Bioaccumulation was modified by target effects of global warming.
Abstract
An increase in the temperature and the acidification of the aquatic environment are among the many consequences of global warming. Climate change can also negatively affect aquatic organisms indirectly, by altering the toxicity of pollutants. Models of climate change impacts on the distribution, fate and ecotoxicity of persistent pollutants are now available. For pharmaceuticals, however, as new environmental pollutants, there are no predictions on this issue. Therefore, this paper organizes the existing knowledge on the effects of temperature, pH and both stressors combined on the toxicity of pharmaceuticals on aquatic organisms. Besides lethal toxicity, the molecular, physiological and behavioral biomarkers of sub-lethal stress were also assessed. Both acute and chronic toxicity, as well as bioaccumulation, were found to be affected. The direction and magnitude of these changes depend on the specific pharmaceutical, as well as the organism and conditions involved. Unfortunately, the response of organisms was enhanced by combined stressors. We compare the findings with those known for persistent organic pollutants, for which the pH has a relatively low effect on toxicity. The acid-base constant of molecules, as assumed, have an effect on the toxicity change with pH modulation. Studies with bivalves have been were overrepresented, while too little attention was paid to producers. Furthermore, the limited number of pharmaceuticals have been tested, and metabolites skipped altogether. Generally, the effects of warming and acidification were rather indicated than explored, and much more attention needs to be given to the ecotoxicology of pharmaceuticals in climate change conditions.
Continue reading ‘Review of warming and acidification effects to the ecotoxicity of pharmaceuticals on aquatic organisms in the era of climate change’Effects of ocean acidification on dopamine-mediated behavioral responses of a coral reef damselfish
Published 6 April 2023 Science ClosedTags: biological response, fish, laboratory, North Atlantic, performance, physiology
Highlights
- CO2-induced ocean acidification (OA) altered dopamine-mediated fish behavior.
- The dopamine D1-receptor agonist SKF 38393 increased anxiety in control fish.
- OA-exposed fish exhibited maximally measurable anxiety levels.
- CO2/pH measured in reef crevasses used as fish shelters were similar to OA tested here.
- The implications of OA on fish fitness should be assessed through future studies.
Abstract
We investigated whether CO2-induced ocean acidification (OA) affects dopamine receptor-dependent behavior in bicolor damselfish (Stegastes partitus). Damselfish were kept in aquaria receiving flow through control (pH ~ 8.03; pCO2 ~ 384 μatm) or OA (pH ~ 7.64; CO2 ~ 1100 μatm) seawater at a rate of 1 L min−1. Despite this relatively fast flow rate, fish respiration further acidified the seawater in both control (pH ~7.88; pCO2 ~ 595 μatm) and OA (pH ~7.55; pCO2 ~ 1450 μatm) fish-holding aquaria. After five days of exposure, damselfish locomotion, boldness, anxiety, and aggression were assessed using a battery of behavioral tests using automated video analysis. Two days later, these tests were repeated following application of the dopamine D1 receptor agonist SKF 38393. OA-exposure induced ceiling anxiety levels that were significantly higher than in control damselfish, and SKF 38393 increased anxiety in control damselfish to a level not significantly different than that of OA-exposed damselfish. Additionally, SKF 38393 decreased locomotion and increased boldness in control damselfish but had no effect in OA-exposed damselfish, suggesting an alteration in activity of dopaminergic pathways that regulate behavior under OA conditions. These results indicate that changes in dopamine D1 receptor function affects fish behavior during exposure to OA. However, subsequent measurements of seawater sampled using syringes during the daytime (~3–4 pm local time) from crevasses in coral reef colonies, which are used as shelter by damselfish, revealed an average pH of 7.73 ± 0.03 and pCO2 of 925.8 ± 62.2 μatm; levels which are comparable to Representative Concentration Pathway (RCP) 8.5 predicted end-of-century mean OA levels in the open ocean. Further studies considering the immediate environmental conditions experienced by fish as well as individual variability and effect size are required to understand potential implications of the observed OA-induced behavioral effects on damselfish fitness in the wild.
Continue reading ‘Effects of ocean acidification on dopamine-mediated behavioral responses of a coral reef damselfish’Balance dysfunction in large yellow croaker in response to ocean acidification
Published 4 April 2023 Science ClosedTags: biological response, fish, laboratory, molecular biology, morphology, performance, physiology
Highlights
- Hearing system is important to soniferous and commercial large yellow croaker.
- How structure and function of whole inner ear responds to acidification is unknow.
- Left and right paired lapillus became asymmetrical after acidification.
- Unable to maintain balance after exposure to higher CO2 acidification
- Nervous system function and mineralization pathways were enriched by RNA-seq.
Abstract
Large yellow croaker (Larimichthys crocea) is a coastal-dwelling soniferous, commercially important fish species that is sensitive to sound. An understanding of how ocean acidification might affect its auditory system is therefore important for its long-term viability and management as a fisheries resource. We tested the effects of ocean acidification with four CO2 treatments (440 ppm (control), 1000 ppm, 1800 ppm, and 3000 ppm) on the inner ear system of this species. After exposure to acidified water for 50 d, the impacts on the perimeter and mass of the sagitta, asteriscus, and lapillus otoliths were determined. In the acidified water treatments, the shape of sagittal otoliths became more irregular, and the surface became rougher. Similar sound frequency ranges triggered startle responses of fish in all treatments. In the highest CO2 treatment (3000 ppm CO2), significant asymmetry of the left and right lapillus perimeter and weight was apparent. Moreover, in the higher CO2 treatments (1800 ppm and 3000 ppm CO2), the fish were unable to maintain a balanced dorsal-up posture and tilted to one side. This result suggested that the balance functions of the inner ear might be affected by ocean acidification, which may threaten large yellow croaker individuals and populations. The molecular response to acidification was analyzed by RNA-Seq. The differentially expressed genes (DEGs) between right and left sensory epithelia of the utricle in each CO2 treatment group were identified. In higher CO2 concentration groups, nervous system function and regulation of bone mineralization pathways were enriched with DEGs. The comparative transcriptome analyses provide valuable molecular information about how the inner ear system responds to an acidified environment.
Continue reading ‘Balance dysfunction in large yellow croaker in response to ocean acidification’Effects of ocean acidification and warming on the specific dynamic action of California Grunion (Leuresthes tenuis) larvae
Published 27 March 2023 Science ClosedTags: biological response, fish, laboratory, multiple factors, North Pacific, physiology, reproduction, temperature
Highlights
- SDA was measured as the difference in metabolic rate of fed and non-fed fish.
- SDA is ∼15% of the daily metabolic energy costs for California Grunion larvae.
- OA conditions shifted the SDA response earlier.
- Changes in SDA with climate can have downstream effects on larval growth.
Abstract
Ocean acidification (OA) and Ocean Warming (OW) are ongoing environmental changes that present a suite of physiological challenges to marine organisms. Larval stages may be especially sensitive to the effects of climate change because the larval phase is a time of critical growth and development. Of particular importance to growth is Specific Dynamic Action (SDA) – the energy used in digestion, absorption, and assimilation of food. Relatively little is known about the energetics of SDA for larval fishes and even less is known about how SDA may be affected by climate change. In this study we used feeding experiments and respirometry assays to characterize the functional form of SDA for California Grunion (Leuresthes tenuis). In a second set of experiments, we tested the independent and combined effects of ocean acidification and warming on SDA. Our first experiment revealed that an elevated metabolic rate was detectable within an hour of feeding, peaked at 3–6 h post feeding, and lasted about 24 h in total. Experiments testing the effects of acidification and warming revealed that temperature generally increased the maximum rate of postprandial respiration and the total amount of energy expended via SDA. In an experiment where feeding level was the same for fish held at different temperatures, elevated pCO2 increased the maximum rate of postprandial respiration and shortened the SDA response. However, in an experiment that allowed fish to consume more food at high temperatures, effects of pCO2 on SDA were minimal. The effects of OA on SDA may depend on a combination of temperature and food availability, and the disruption of SDA with OA may be part of a chain of events where digestion and assimilation efficiency are impaired with potential consequences for growth, survival, and population replenishment.
Continue reading ‘Effects of ocean acidification and warming on the specific dynamic action of California Grunion (Leuresthes tenuis) larvae’Sensitivity of fishery resources to climate change in the warm-temperate Southwest Atlantic Ocean
Published 24 March 2023 Science ClosedTags: biological response, fish, fisheries, individualmodeling, modeling, multiple factors, oxygen, South Atlantic, temperature
Climate change impacts on fishery resources have been widely reported worldwide. Nevertheless, a knowledge gap remains for the warm-temperate Southwest Atlantic Ocean—a global warming hotspot that sustains important industrial and small-scale fisheries. By combining a trait-based framework and long-term landing records, we assessed species’ sensitivity to climate change and potential changes in the distribution of important fishery resources (n = 28; i.e., bony fishes, chondrichthyans, crustaceans, and mollusks) in Southern Brazil, Uruguay, and the northern shelf of Argentina. Most species showed moderate or high sensitivity, with mollusks (e.g., sedentary bivalves and snails) being the group with the highest sensitivity, followed by chondrichthyans. Bony fishes showed low and moderate sensitivities, while crustacean sensitivities were species-specific. The stock and/or conservation status overall contributed the most to higher sensitivity. Between 1989 and 2019, species with low and moderate sensitivity dominated regional landings, regardless of the jurisdiction analyzed. A considerable fraction of these landings consisted of species scoring high or very high on an indicator for potential to change their current distribution. These results suggest that although the bulk of past landings were from relatively climate-resilient species, future catches and even entire benthic fisheries may be jeopardized because (1) some exploited species showed high or very high sensitivities and (2) the increase in the relative representation of landings in species whose distribution may change. This paper provides novel results and insights relevant for fisheries management from a region where the effects of climate change have been overlooked, and which lacks a coordinated governance system for climate-resilient fisheries.
Continue reading ‘Sensitivity of fishery resources to climate change in the warm-temperate Southwest Atlantic Ocean’Swimming performance of sharks and rays under climate change
Published 21 March 2023 Science ClosedTags: biological response, fish, morphology, multiple factors, performance, physiology, review, temperature
Climate change stressors (e.g., warming and ocean acidification) are an imminent challenge to the physiological performance of marine organisms. Several studies spanning the last decade have reported widespread effects of warming and acidification on marine fishes, especially teleosts, but more work is needed to elucidate the responses in marine elasmobranchs, i.e., sharks and rays. Dispersal capacity, as a result of locomotor performance, is a crucial trait that will determine which group of elasmobranchs will be more or less vulnerable to changes in the environment. In fact, efficient and high locomotor performance may determine the capacity for elasmobranchs to relocate to a more favorable area. In this review we integrate findings from work on locomotion of marine sharks and rays to identify characteristics that outline potential vulnerabilities and strength of sharks and rays under climate change. Traits such as intraspecific variability in response to climatic stressors, wide geographic range, thermotaxis, fast swimming or low energetic costs of locomotion are likely to enhance the capacity to disperse. Future studies may focus on understanding the interacting effect of climatic stressors on morphology, biomechanics and energetics of steady and unsteady swimming, across ontogeny and species.
Continue reading ‘Swimming performance of sharks and rays under climate change’Elevated CO2 levels did not induce species- or tissue-specific damage in young-of-year salmonids
Published 14 March 2023 Science ClosedTags: biological response, fish, laboratory, morphology, reproduction
There are few studies that assess CO2 effects on fish tissues. To study these effects, young-of-year Arctic Charr (Salvelinus alpinus), Rainbow Trout (Oncorhynchus mykiss), and Brook Charr (S. fontinalis) were exposed to either control levels of CO2 (1,400 μatm) or elevated levels of CO2 (5,236 μatm) for 15 days. Fish were then sampled for gill, liver, and heart tissues and histologically analyzed. A species effect was observed for the length of secondary lamellae, as Arctic Charr had significantly shorter secondary lamellae than the other species. No notable changes within the gills and livers of Arctic Charr, Brook Charr, or Rainbow Trout exposed to elevated CO2 were observed. Generally, our results indicated that elevated CO2 levels over 15 days do not induce catastrophic tissue damage and it is unlikely that fish health would be seriously impacted. Ongoing research dedicated to examining how elevated CO2 long-term may affect internal tissues of fish will allow for a more comprehensive understanding of how fish may fair with ongoing climate change and in aquaculture facilities.
Continue reading ‘Elevated CO2 levels did not induce species- or tissue-specific damage in young-of-year salmonids’Oceans and the changing climate
Published 8 March 2023 Science ClosedTags: biological response, fish, fisheries, review
Increasing levels of atmospheric greenhouse gases are producing changes in the world’s oceans and coastal environments, such as increasing sea surface temperatures, ocean acidification, and rising sea levels. This chapter explores the human dimensions of three climate change impacts: (1) rising sea levels, measures to adapt, and the potential displacement of persons from eroding, low-lying coastal areas; (2) the migration of fish stocks to new habitats resulting from increasing seawater temperatures; and (3) the degradation of coral reefs and impacts to shellfish resulting from ocean acidification. With sea level rise, millions of people in low-lying coastal cities and small island developing states must adapt or be displaced, and some will become climate refugees. In the case of fisheries, distributions of some fish stocks are already changing because of increasing ocean temperatures. These shifts have great implications for both fishers and managers of marine resources. Finally, rising atmospheric concentrations of CO2 that lower the ocean’s pH make it more difficult for corals and shellfish to precipitate the calcium carbonate that forms their exoskeletons and shells, affecting users of tropical coral reef ecosystems as well as the shellfish aquaculture industry.
Continue reading ‘Oceans and the changing climate’Effects of elevated CO2 on metabolic rate and nitrogenous waste handling in the early life stages of yellowfin tuna (Thunnus albacares)
Published 6 March 2023 Science ClosedTags: biological response, fish, laboratory, physiology, reproduction
Highlights
- Little is known about how tuna species will respond to ocean acidification (OA).
- CO2 altered nitrogenous waste excretion and metabolic rate in yolk sac larvae.
- CO2 did not change yolk sac depletion in embryos.
- CO2 did not alter nitrogen accumulation in yellowfin tuna.
- Yellowfin tuna were more robust to CO2 than predicted.
Abstract
Ocean acidification is predicted to have a wide range of impacts on fish, but there has been little focus on broad-ranging pelagic fish species. Early life stages of fish are thought to be particularly susceptible to CO2 exposure, since acid-base regulatory faculties may not be fully developed. We obtained yellowfin tuna (Thunnus albacares) from a captive spawning broodstock population and exposed them to control or 1900 μatm CO2 through the first three days of development as embryos transitioned into yolk sac larvae. Metabolic rate, yolk sac depletion, and oil globule depletion were measured to assess overall energy usage. To determine if CO2 altered protein catabolism, tissue nitrogen content and nitrogenous waste excretion were quantified. CO2 exposure did not significantly impact embryonic metabolic rate, yolk sac depletion, or oil globule depletion, however, there was a significant decrease in metabolic rate at the latest measured yolk sac larval stage (36 h post fertilization). CO2-exposure led to a significant increase in nitrogenous waste excretion in larvae, but there were no differences in nitrogen tissue accumulation. Nitrogenous waste accumulated in embryos as they developed but decreased after hatch, coinciding with a large increase in nitrogenous waste excretion and increased metabolic rate in newly hatched larvae. Our results provide insight into how yellowfin tuna are impacted by increases in CO2 in early development, but more research with higher levels of replication is needed to better understand long-term impacts and acid-base regulatory mechanisms in this important pelagic fish.
Continue reading ‘Effects of elevated CO2 on metabolic rate and nitrogenous waste handling in the early life stages of yellowfin tuna (Thunnus albacares)’The effects of ocean acidification on fishes – history and future outlook
Published 1 March 2023 Science ClosedTags: biological response, fish, review
The effects of increased levels of carbon dioxide (CO2) on the Earth’s temperature have been known since the end of the 19th century. It was long believed that the oceans’ buffering capacity would counteract any effects of dissolved CO2 in marine environments, but during recent decades, many studies have reported detrimental effects of ocean acidification on aquatic organisms. The most prominent effects can be found within the field of behavioural ecology, e.g., complete reversal of predator avoidance behaviour in CO2-exposed coral reef fish. Some of the studies have been very influential, receiving hundreds of citations over recent years. The results have also been conveyed to policymakers and publicized in prominent media outlets for the general public. Those extreme effects of ocean acidification on fish behaviour have, however, spurred controversy, given that more than a century of research suggests that there are few or no negative effects of elevated CO2 on fish physiology. This is due to sophisticated acid–base regulatory mechanisms that should enable their resilience to near-future increases in CO2. In addition, an extreme “decline effect” has recently been shown in the literature regarding ocean acidification and fish behaviour, and independent research groups have been unable to replicate some of the most profound effects. Here, the author presents a brief historical overview on the effects of elevated CO2 and ocean acidification on fishes. This historical recap is warranted because earlier work, prior to a recent (c. 10 year) explosion in interest, is often overlooked in today’s ocean acidification studies, despite its value to the field. Based on the historical data and the current knowledge status, the author suggests future strategies with the aim to improve research rigour and clarify the understanding of the effects of ocean acidification on fishes.
Continue reading ‘The effects of ocean acidification on fishes – history and future outlook’
