Carbon dioxide levels in many estuaries fluctuate and, in several cases, reach extremes much higher than those predicted for oceans by the end of the century. Moreover, estuaries are characterized by natural fluctuations in salinity, and reduced pH, from increased pCO2, exposes estuarine organisms to multiple stresses. Although the effects of low pH on the reproduction of several marine copepod species have been assessed, studies examining effects of pH in estuarine copepod species are extremely scarce. Here, we aim at understanding the reproductive response of Gladioferens pectinatus to the stress posed by both salinity and pH. G. pectinatus was exposed to salinities 2 and 10, at four different pH levels each. Our results show no impairment in the brood size, embryonic development time and hatching success under low pH levels at either salinities. However, at salinity 2, the percentage of nauplii growing into adults significantly decreased at low pH, whereas at salinity 10, no major effect was observed. We argue that the combination of osmoregulation and acidity induced stress response can affect the development of nauplii and copepodites, as well as adult recruitment, likely due to energy reallocation and molting impairment. We also argue that resilience and phenotypic plasticity highly influence the ability of different copepod species and populations to reproduce and grow under stressful combinations of environmental parameters. This study points out the importance of understanding the effects of multiple stresses or parameters on the adaptability of organisms to water acidification.
Posts Tagged 'salinity'
A combination of salinity and pH affects the recruitment of Gladioferens pectinatus (Brady) (Copepoda; Calanoida)Published 18 April 2017 Science Leave a Comment
Tags: biological response, crustaceans, laboratory, morphology, multiple factors, physiology, reproduction, salinity, zooplankton
The effects of salinity and pH on fertilization, early development, and hatching in the crown-of-thorns seastarPublished 28 February 2017 Science Leave a Comment
Tags: biological response, echinoderms, laboratory, morphology, multiple factors, reproduction, salinity, South Pacific
Understanding the influence of environmental factors on the development and dispersal of crown-of-thorns seastars is critical to predicting when and where outbreaks of these coral-eating seastars will occur. Outbreaks of crown-of-thorns seastars are hypothesized to be driven by terrestrial runoff events that increase nutrients and the phytoplankton food for the larvae. In addition to increasing larval food supply, terrestrial runoff may also reduce salinity in the waters where seastars develop. We investigated the effects of reduced salinity on the fertilization and early development of seastars. We also tested the interactive effects of reduced salinity and reduced pH on the hatching of crown-of-thorns seastars. Overall, we found that reduced salinity has strong negative effects on fertilization and early development, as shown in other echinoderm species. We also found that reduced salinity delays hatching, but that reduced pH, in isolation or in combination with lower salinity, had no detectable effects on this developmental milestone. Models that assess the positive effects of terrestrial runoff on the development of crown-of-thorns seastars should also consider the strong negative effects of lower salinity on early development including lower levels of fertilization, increased frequency of abnormal development, and delayed time to hatching.
Adult exposure to ocean acidification is maladaptive for larvae of the Sydney rock oyster Saccostrea glomerata in the presence of multiple stressorsPublished 16 February 2017 Science Leave a Comment
Tags: biological response, laboratory, mollusks, morphology, mortality, multiple factors, nutrients, physiology, salinity, South Pacific, temperature
Parental effects passed from adults to their offspring have been identified as a source of rapid acclimation that may allow marine populations to persist as our surface oceans continue to decrease in pH. Little is known, however, whether parental effects are beneficial for offspring in the presence of multiple stressors. We exposed adults of the oyster Saccostrea glomerata to elevated CO2 and examined the impacts of elevated CO2 (control = 392; 856 µatm) combined with elevated temperature (control = 24; 28°C), reduced salinity (control = 35; 25) and reduced food concentration (control = full; half diet) on their larvae. Adult exposure to elevated CO2 had a positive impact on larvae reared at elevated CO2 as a sole stressor, which were 8% larger and developed faster at elevated CO2 compared with larvae from adults exposed to ambient CO2. These larvae, however, had significantly reduced survival in all multistressor treatments. This was particularly evident for larvae reared at elevated CO2 combined with elevated temperature or reduced food concentration, with no larvae surviving in some treatment combinations. Larvae from CO2-exposed adults had a higher standard metabolic rate. Our results provide evidence that parental exposure to ocean acidification may be maladaptive when larvae experience multiple stressors.
Plasticity and inter-population variability in physiological and life-history traits of the mussel Mytilus chilensis: A reciprocal transplant experimentPublished 13 February 2017 Science Leave a Comment
Tags: biological response, calcification, field, molecular biology, mollusks, morphology, multiple factors, physiology, salinity
Geographically widespread species must cope with environmental differences between habitats. Information concerning geographic variations in response to climate variability is critical because many morphological, life-history and physiological traits show variation across space. Reciprocal transplant experiments have shown to be relevant to evaluate the role of phenotypic plasticity and potential local adaptation in ecophysiological responses when coping with environmental variability. In this study, we characterize through reciprocal transplant experiments the reaction norms of morphological, biochemical, physiological and life-history traits between two intertidal populations of the socioeconomically important mussel Mytilus chilensis, inhabiting contrasting local environments (estuarine vs coastal habitats). We found a gradient in phenotypic plasticity with plastic trait responses in metabolic, ingestion and clearance rates, and in HsP70 gene expression, and some traits with responses more canalized as growth and calcification rates. This emphasizes that responses not only vary across different local populations but also in different traits in M. chilensis, thus it is difficult to establish an overall trend of the responses at integrated organismal level. Moreover, the synergistic interaction of factors such as salinity and carbonate system parameters evaluated make it necessary to study the response at the population level with emphasis on benthic species important in aquaculture. Finally, field studies such as this one are useful for documenting the patterns of traits variation that occur in nature, identifying possible causes of such variation, and generating testable hypotheses for future controlled experiments.
Environmental drivers of coccolithophore abundance and calcification across Drake Passage (Southern Ocean) (update)Published 2 November 2016 Science Leave a Comment
Tags: abundance, Antarctic, biological response, BRcommunity, calcification, chemistry, community composition, field, light, morphology, multiple factors, nutrients, otherprocess, phytoplankton, primary production, salinity, temperature
Although coccolithophores are not as numerically common or as diverse in the Southern Ocean as they are in subpolar waters of the North Atlantic, a few species, such as Emiliania huxleyi, are found during the summer months. Little is actually known about the calcite production (CP) of these communities or how their distribution and physiology relate to environmental variables in this region. In February 2009, we made observations across Drake Passage (between South America and the Antarctic Peninsula) of coccolithophore distribution, CP, primary production, chlorophyll a and macronutrient concentrations, irradiance and carbonate chemistry. Although CP represented less than 1 % of total carbon fixation, coccolithophores were widespread across Drake Passage. The B/C morphotype of E. huxleyi was the dominant coccolithophore, with low estimates of coccolith calcite (∼ 0.01 pmol C coccolith−1) from biometric measurements. Both cell-normalised calcification (0.01–0.16 pmol C cell−1 d−1) and total CP (< 20 µmol C m−3 d−1) were much lower than those observed in the subpolar North Atlantic where E. huxleyi morphotype A is dominant. However, estimates of coccolith production rates were similar (0.1–1.2 coccoliths cell−1 h−1) to previous measurements made in the subpolar North Atlantic. A multivariate statistical approach found that temperature and irradiance together were best able to explain the observed variation in species distribution and abundance (Spearman’s rank correlation ρ = 0.4, p < 0.01). Rates of calcification per cell and coccolith production, as well as community CP and E. huxleyi abundance, were all positively correlated (p < 0.05) to the strong latitudinal gradient in temperature, irradiance and calcite saturation states across Drake Passage. Broadly, our results lend support to recent suggestions that coccolithophores, especially E. huxleyi, are advancing polewards. However, our in situ observations indicate that this may owe more to sea-surface warming and increasing irradiance rather than increasing CO2 concentrations.
Tags: biological response, fish, growth, laboratory, mortality, multiple factors, physiology, salinity
Climate change with concomitant ocean acidification presents a problem to coastal ecosystems, including estuaries. It is well-documented that fish growth, development, and survival are dependent on environmental factors such as temperature and salinity. Considering the economic and recreational importance of red drum (Sciaenops ocellatus), it is important to understand both acute and long-term effects of environmental change on juveniles released into native waters as part of stock enhancement programs. Experiments were designed to compare survival, growth and body composition of juvenile red drum grown under different salinity and pH treatments.
Research was conducted in a closed recirculating system with juvenile red drum (42±9.9 mm) randomly stocked at a density of 13 fish/tank and fed daily to satiation (∼6% body weight). Fish were subjected to salinity treatments of 40 or 30 and a pH of either7.5, 8.1, 8.5, or 9.0 (n = 8 replicates per treatment). Each trial was conducted for 14 days.
Results show that at a salinity of 40, there was a significant difference between survival of the juvenile red drum at pH 7.5 and 9.0 (p=0.03). Survival was not significant between pH levels at salinity of 30. Results indicated pH had no significant effect on specific growth rate (SGR, p ≥ 0.05); however, increased salinity significantly decreased growth (p< 0.05), and there was a significant interaction between pH and salinity. There was no significant impact from pH or salinity on protein retention (p≥0.05). At 40 the whole body ash increased as pH increased (p=0.003). Ash was also significantly different between pH values at salinity 30 (p=0.02). Whole body energy was not significantly affected by pH (p ≥ 0.05); but increased salinity caused a significant decrease in energy retention (p < 0.05), and there was no significant interaction between pH and salinity affecting energy retention.
These results indicate that salinity is a more critical factor to consider than pH when engaging in stock enhancement efforts, especially at high salinity. The results of this study will help fisheries managers increase the rate of survival of hatchery-reared red drum when released into the wild. The results indicate that research should be conducted to investigate the effects of hypersalinity (>40) and pH on juvenile red drum as related to growth rates, and growth hormone production. Future research should also focus on the long term effects of low and high pH exposure of juveniles to adult life stages, and should examine otolith development, behavior and the effects on reproduction of red drum.
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressorsPublished 7 July 2016 Science Leave a Comment
Tags: biological response, laboratory, molecular biology, mollusks, morphology, multiple factors, North Pacific, physiology, salinity, temperature
The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs.