Archive for January, 2021



Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece

Although the rise of antibiotic and multidrug resistant bacteria is one of the biggest current threats to human health, our understanding of the mechanisms involved in antibiotic resistance selection remains scarce. We performed whole genome sequencing of 21 Pseudomonas strains, previously isolated from an active submarine volcano of Greece, the Kolumbo volcano. Our goal was to identify the genetic basis of the enhanced co-tolerance to antibiotics and acidity of these Pseudomonas strains. Pangenome analysis identified 10,908 Gene Clusters (GCs). It revealed that the numbers of phage-related GCs and sigma factors, which both provide the mechanisms of adaptation to environmental stressors, were much higher in the high tolerant Pseudomonas strains compared to the rest ones. All identified GCs of these strains were associated with antimicrobial and multidrug resistance. The present study provides strong evidence that the CO2-rich seawater of the volcano associated with low pH might be a reservoir of microorganisms carrying multidrug efflux-mediated systems and pumps. We, therefore, suggest further studies of other extreme environments (or ecosystems) and their associated physicochemical parameters (or factors) in the rise of antibiotic resistance.

Continue reading ‘Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece’

Fish sex organs boosted under high-CO2

Research from the University of Adelaide has found that some species of fish will have higher reproductive capacity because of larger sex organs, under the more acidic oceans of the future.

Published in PLOS Biology, the researchers say that far from the negative effects expected under the elevated CO2  levels in our oceans predicted for the end of the century, these fish capitalise on changes to the underwater ecosystems to produce more sperm and eggs. They also look after them better, enhancing the chances of reproductive success.

“The warming oceans absorb about one-third of the additional CO2 being released into the atmosphere from carbon emissions, causing the oceans to acidify,” says lead author Professor Ivan Nagelkerken from the University’s Environment Institute and Southern Seas Ecology Laboratories.

“We know that many species are negatively affected in their behaviour and physiology by ocean acidification. But we found that in this species of temperate fish – the common triplefin – both males and females had larger gonads under conditions of ocean acidification. This meant increased egg and sperm production and therefore more offspring.”

Continue reading ‘Fish sex organs boosted under high-CO2’

Ocean acidification boosts reproduction in fish via indirect effects

Ocean acidification affects species populations and biodiversity through direct negative effects on physiology and behaviour. The indirect effects of elevated CO2 are less well known and can sometimes be counterintuitive. Reproduction lies at the crux of species population replenishment, but we do not know how ocean acidification affects reproduction in the wild. Here, we use natural CO2 vents at a temperate rocky reef and show that even though ocean acidification acts as a direct stressor, it can indirectly increase energy budgets of fish to stimulate reproduction at no cost to physiological homeostasis. Female fish maintained energy levels by compensation: They reduced activity (foraging and aggression) to increase reproduction. In male fish, increased reproductive investment was linked to increased energy intake as mediated by intensified foraging on more abundant prey. Greater biomass of prey at the vents was linked to greater biomass of algae, as mediated by a fertilisation effect of elevated CO2 on primary production. Additionally, the abundance and aggression of paternal carers were elevated at the CO2 vents, which may further boost reproductive success. These positive indirect effects of elevated CO2 were only observed for the species of fish that was generalistic and competitively dominant, but not for 3 species of subordinate and more specialised fishes. Hence, species that capitalise on future resource enrichment can accelerate their reproduction and increase their populations, thereby altering species communities in a future ocean.

Continue reading ‘Ocean acidification boosts reproduction in fish via indirect effects’

Some fish develop larger sex organs when CO2 levels are high

A new study from the University of Adelaide has revealed that some fish develop larger sex organs when they are exposed to high levels of CO2.

A new study from the University of Adelaide has revealed that some fish develop larger sex organs when they are exposed to high levels of CO2. The experts report that the reproductive capacity of these fish will increase as the oceans continue to absorb carbon emissions.

The researchers said that far from the negative effects expected under the elevated CO2 levels in our oceans predicted for the end of the century, these fish capitalize on changes to the underwater ecosystems to produce more sperm and eggs.

“The warming oceans absorb about one-third of the additional CO2 being released into the atmosphere from carbon emissions, causing the oceans to acidify,” said study lead author Professor Ivan Nagelkerken.

Continue reading ‘Some fish develop larger sex organs when CO2 levels are high’

Effects of ocean acidification on growth, pigment contents and antioxidant potential of the subtropical Atlantic red alga Hypnea pseudomusciformis Nauer, Cassano & M.C. Oliveira (Gigartinales) in laboratory

Marine ecosystems are subject to several modifications due to anthropogenic impacts, including ocean acidification caused by the absorption of excessive CO2 present in the atmosphere. Perspectives are for dramatic modifications in seawater pH and more than 60% of the ocean surface impacted over the next 100 years by global change. In this study, ocean acidification scenarios were simulated by CO2 enrichment into seawater in three pH levels (8.0, 7.6 and 7.2) using a bioreactor system in laboratory conditions. Experimental evaluation was performed with Hypnea pseudomusciformis Nauer, Cassano & M.C. Oliveira due to its great importance in coastal marine ecosystems for primary production and commercial interest. Contrary to our initial hypothesis, the growth rate of H. pseudomusciformis decreased significantly with decreased pH conditions, even with increased availability of CO2. The maximum quantum yield and chlorophyll a content were also negatively affected by the pH reduction, while an increase in antioxidant activity was observed, indicating physiological stress. The physiological responses to decreased pH conditions reflect the importance of species-level studies and corroborate the changes caused by the ocean acidification on the macroalgal species.

Continue reading ‘Effects of ocean acidification on growth, pigment contents and antioxidant potential of the subtropical Atlantic red alga Hypnea pseudomusciformis Nauer, Cassano & M.C. Oliveira (Gigartinales) in laboratory’

Effects of ocean warming and acidification on diatom silicate formation

We invite applications for a fully funded PhD position on a three-year project to assess the impact of ocean warming and acidification on the general health, macromolecular composition, silicate formation and sinking rates of diatoms. Diatoms are one of the most diverse and ecologically important group of microalgae in the ocean, whose growth and silicification (cell-wall formation using silica) is modulated by their environment. Understanding to what extent growth and silicification are stimulated or inhibited by concurrent changes in temperature and ocean acidification, as a result of increasing atmospheric CO2, is needed to enable reliable predictions on the future role of diatoms in ocean food webs and biogeochemistry. Through a combination of laboratory and field studies (including algae culturing and incubation experiments, chemical analysis, FTIR-microspectroscopy as well as ship expeditions for sampling and on-board experiments), the candidate will explore the physiological response of diatoms to future ocean conditions and be responsible for collecting data that will be used to model projections of these changes to predict how ongoing global warming will change ocean ecology and biogeochemistry.

Continue reading ‘Effects of ocean warming and acidification on diatom silicate formation’

Effect of CO2 driven ocean acidification on calcification, physiology and ovarian cells of tropical sea urchin Salmacis virgulata – a microcosm approach

In the present study, we depict the structural modification of test minerals, physiological response and ovarian damage in the tropical sea urchin Salmacis virgulata using microcosm CO2 (Carbon dioxide) perturbation experiment. S. virgulata were exposed to hypercapnic conditions with four different pH levels using CO2 gas bubbling method that reflects ambient level (pH 8.2) and elevated pCO2 scenarios (pH 8.0, 7.8 and 7.6). The variations in physical strength and mechanical properties of S. virgulata test were evaluated by thermogravimetric analysis, Fourier transform infrared spectroscopy, X-ray diffraction analysis and scanned electron microscopy analysis. Biomarker enzymes such as glutathione-S-transferase, catalase, acetylcholine esterase, lipid peroxidase and reduced glutathione showed physiological stress and highly significant (p < 0.01) towards pH 7.6 and 7.8 treatments. Ovarian cells were highly damaged at pH 7.6 and 7.8 treatments. This study proved that the pH level 7.6 and 7.8 drastically affect calcification, physiological response and ovarian cells in S. virgulata.

Continue reading ‘Effect of CO2 driven ocean acidification on calcification, physiology and ovarian cells of tropical sea urchin Salmacis virgulata – a microcosm approach’

Impacts of acclimation in warm-low pH conditions on the physiology of the sea urchin Heliocidaris erythrogramma and carryover effects for juvenile offspring

Ocean warming (OW) and acidification (OA) affects nearly all aspects of marine organism physiology and it is important to consider both stressors when predicting responses to climate change. We investigated the effects of long-term exposure to OW and OA on the physiology of adults of the sea urchin, Heliocidaris erythrogramma, a species resident in the southeast Australia warming hotspot. The urchins were slowly introduced to stressor conditions in the laboratory over a 7-week adjustment period to three temperature (ambient, +2°C, +3°C) and two pH (ambient: pHT 8.0; −0.4 units: pHT 7.6) treatments. They were then maintained in a natural pattern of seasonal temperature and photoperiod change, and fixed pH, for 22 weeks. Survival was monitored through week 22 and metabolic rate was measured at 4 and 12 weeks of acclimation, feeding rate and ammonia excretion rate at 12 weeks and assimilation efficiency at 13 weeks. Acclimation to +3°C was deleterious regardless of pH. Mortality from week 6 indicated that recent marine heatwaves are likely to have been deleterious to this species. Acclimation to +2°C did not affect survival. Increased temperature decreased feeding and increased excretion rates, with no effect of acidification. While metabolic rate increased additively with temperature and low pH at week 4, there was no difference between treatments at week 12, indicating physiological acclimation in surviving urchins to stressful conditions. Regardless of treatment, H. erythrogramma had a net positive energy budget indicating that the responses were not due to energy limitation. To test for the effect of parental acclimation on offspring responses, the offspring of acclimated urchins were reared to the juvenile stage in OW and OA conditions. Parental acclimation to warming, but not acidification altered juvenile physiology with an increase in metabolic rate. Our results show that incorporation of gradual seasonal environmental change in long-term acclimation can influence outcomes, an important consideration in predicting the consequences of changing climate for marine species.

Continue reading ‘Impacts of acclimation in warm-low pH conditions on the physiology of the sea urchin Heliocidaris erythrogramma and carryover effects for juvenile offspring’

Short-term response of flat tree oyster, Isognomon alatus to CO2 acidified seawater in laboratory and field experiments

Seawater changing chemistry has consequences on coastal ecosystems and their living resources. Future projections suggest the pH could drop ~0.2-0.3 pH units by the year 2100 under a business-as-usual (BAU) CO2 emission scenario. Marine calcifying organisms such as corals, calcifying algae, crustaceans, mussels, oysters and clams are most likely to be impacted by ocean acidification. The Isognomon alatus (flat tree oyster) is an important species that can be negatively affected by the lowering of seawater pH. Isognomon alatus is an important food source, a substrate for other benthic organisms (e.g., stone crab, Menippe mercenaria) and contribute to nutrients recycling in coastal ecosystems. The study was conducted to test the impacts acidified seawater CO2 on the growth of I. alatus under controlled laboratory conditions as well as field experiment. The Isognomon alatus lost weight and experienced negative growth rates of –0.56 ± 0.36 mg g-1day-1 under average pH values of 7.8 expected by the end of this century compared to a loss of –0.26 ± 0.23 mg g-1day-1 under ambient pH (value 8.1) conditions. In contrast, I. alatus incubated in a field experiment showed a gain in weight and positive growth of 3.30 ± 0.23 mg g-1day-1 despite exposure to pH levels (~7.4) during low tide significantly lower than those experienced in the laboratory. Overall, the results showed concern on the impacts of acidification flat tree oyster (Bivalvia:Isognomonidae). A decline of calcifying bivalves populations can impact coastal ecosystems function and indirectly affect the human beings that depend on them as a food source. 

Continue reading ‘Short-term response of flat tree oyster, Isognomon alatus to CO2 acidified seawater in laboratory and field experiments’

Technical webinar: “HKIE MMNC X HKU marine conservation 3rd session – ocean acidification”

Date, time & venue

2021-01-22;7:30pm to 8:30pm;Online Webinar by Zoom

Programme Highlights

In this webinar, we are grateful to invite Miss Tiffany Fung, Research Assistant from The University of Hong Kong, to be our speaker for “HKIE MMNC X HKU Marine Conservation 3rd Session – Ocean Acidification”. Through the series of webinar, HKIE MMNC division would like to widen our member’s knowledge of Marine Conservation so as to be aware of importance of environmental protection.

Continue reading ‘Technical webinar: “HKIE MMNC X HKU marine conservation 3rd session – ocean acidification”’

Research scientist 3 marine carbon

The Cooperative Institute for Climate, Ocean, and Ecosystem Studies (CICOES) has existed since 1977 for the purpose of fostering research collaboration between UW and the National Oceanic and Atmospheric Administration (NOAA). CICOES’s research is at the forefront of investigations on climate change, ocean acidification, fisheries assessments, and tsunami forecasting.

CICOES has an outstanding opportunity for a Research Scientist in Marine Carbon. This position will support various ongoing research projects associated with marine carbon chemistry, specifically local and global efforts to quantify ocean carbon cycling and ocean acidification.

Continue reading ‘Research scientist 3 marine carbon’

Coccolithophore calcification studied by single-cell impedance cytometry: towards single-cell PIC:POC measurements

Since the industrial revolution 30% of the anthropogenic CO2 is absorbed by oceans, resulting in ocean acidification, which is a threat to calcifying algae. As a result, there has been profound interest in the study of calcifying algae, because of their important role in the global carbon cycle. The coccolithophore Emiliania huxleyi is considered to be globally the most dominant calcifying algal species, which creates a unique exoskeleton from inorganic calcium carbonate platelets. The PIC (particulate inorganic carbon): POC (particulate organic carbon) ratio describes the relative amount of inorganic carbon in the algae and is a critical parameter in the ocean carbon cycle.

In this research we explore the use of microfluidic single-cell impedance spectroscopy in the field of calcifying algae. Microfluidic impedance spectroscopy enables us to characterize single-cell electrical properties in a noninvasive and label-free way. We use the ratio of the impedance at high frequency vs. low frequency, known as opacity, to discriminate between calcified coccolithophores and coccolithophores with a calcite exoskeleton dissolved by acidification (decalcified).

We have demonstrated that using opacity we can discriminate between calcified and decalcified coccolithophores with an accuracy of 94.1%. We have observed a correlation between the measured opacity and the cell height in the channel, which is supported by FEM simulations. The difference in cell density between calcified and decalcified cells can explain the difference in cell height and therefore the measured opacity.

Continue reading ‘Coccolithophore calcification studied by single-cell impedance cytometry: towards single-cell PIC:POC measurements’

Sea‐ice microbial communities in the Central Arctic Ocean: limited responses to short‐term pCO2 perturbations

The Arctic Ocean is more susceptible to ocean acidification than other marine environments due to its weaker buffering capacity, while its cold surface water with relatively low salinity promotes atmospheric CO2 uptake. We studied how sea‐ice microbial communities in the central Arctic Ocean may be affected by changes in the carbonate system expected as a consequence of ocean acidification. In a series of four experiments during late summer 2018 aboard the icebreaker Oden, we addressed microbial growth, production of dissolved organic carbon (DOC) and extracellular polymeric substances (EPS), photosynthetic activity, and bacterial assemblage structure as sea‐ice microbial communities were exposed to elevated partial pressures of CO2 (pCO2). We incubated intact, bottom ice‐core sections and dislodged, under‐ice algal aggregates (dominated by Melosira arctica) in separate experiments under approximately 400, 650, 1000, and 2000 μatm pCO2 for 10 d under different nutrient regimes. The results indicate that the growth of sea‐ice algae and bacteria was unaffected by these higher pCO2 levels, and concentrations of DOC and EPS were unaffected by a shifted inorganic C/N balance, resulting from the CO2 enrichment. These central Arctic sea‐ice microbial communities thus appear to be largely insensitive to short‐term pCO2 perturbations. Given the natural, seasonally driven fluctuations in the carbonate system of sea ice, its resident microorganisms may be sufficiently tolerant of large variations in pCO2 and thus less vulnerable than pelagic communities to the impacts of ocean acidification, increasing the ecological importance of sea‐ice microorganisms even as the loss of Arctic sea ice continues.

Continue reading ‘Sea‐ice microbial communities in the Central Arctic Ocean: limited responses to short‐term pCO2 perturbations’

How tropical corals cope with climate change

© Pixabay

A laboratory analysis of two tropical coral species reveals the mechanism that they use to offset the combined effects of heat stress and ocean acidification1 ­– effects known to disrupt coral skeletal growth.

The photosynthetic algae living inside the corals supply energy to maintain the processes that contribute to their skeletal growth, a team of researchers has found. This knowledge will help them understand how the corals cope with the increased carbon dioxide levels that make the oceans warmer and more acidic.

Heat stress bleaches corals. However, no previous studies had examined how temperature and ocean acidification affect the corals, particularly their skeletal growth.

Continue reading ‘How tropical corals cope with climate change’

Climate change alters the haemolymph microbiome of oysters

Highlights

  • Elevated pCO2 and temperature caused shifts in the oyster haemolymph microbiome.
  • Elevated pCO2 was the strongest driver of species diversity and richness.
  • Elevated pCO2 and temperature caused a loss of “core” bacteria.
  • There was no evidence for a shift in the microbiome from a mutualistic to pathogenic state.

Abstract

The wellbeing of marine organisms is connected to their microbiome. Oysters are a vital food source and provide ecological services, yet little is known about how climate change such as ocean acidification and warming will affect their microbiome. We exposed the Sydney rock oyster, Saccostrea glomerata, to orthogonal combinations of temperature (24, 28 °C) and pCO2 (400 and 1000 μatm) for eight weeks and used amplicon sequencing of the 16S rRNA (V3-V4) gene to characterise the bacterial community in haemolymph. Overall, elevated pCO2 and temperature interacted to alter the microbiome of oysters, with a clear partitioning of treatments in CAP ordinations. Elevated pCO2 was the strongest driver of species diversity and richness and elevated temperature also increased species richness. Climate change, both ocean acidification and warming, will alter the microbiome of Sglomerata which may increase the susceptibility of oysters to disease.

Continue reading ‘Climate change alters the haemolymph microbiome of oysters’

Late afternoon seasonal transition to dissolution in a coral reef: an early warning of a net dissolving ecosystem?

There are concerns that reefs will transition from net calcifying to net dissolving in the near future due to decreasing calcification and increasing dissolution rates. Here we present in situ rates of net ecosystem calcification (NEC) and net ecosystem production (NEP) on a coral reef flat using a slack‐water approach. Up until dusk, the reef was net calcifying in most months but shifted to net dissolution in austral summer, coinciding with high respiration rates and a lower aragonite saturation state (Ωarag). The estimated sediment contribution to NEC ranged from 8 – 21 % during the day and 45 – 78 % at night, indicating that high rates of sediment dissolution may cause the transition to reef dissolution. This late afternoon seasonal transition to negative NEC may be an early warning sign of the reef shifting to a net dissolving state and may be occurring on other reefs.

Continue reading ‘Late afternoon seasonal transition to dissolution in a coral reef: an early warning of a net dissolving ecosystem?’

Greater mitochondrial energy production provides resistance to ocean acidification in “winning” hermatypic corals

Coral communities around the world are projected to be negatively affected by ocean acidification. Not all coral species will respond in the same manner to rising CO2 levels. Evidence from naturally acidified areas such as CO2 seeps have shown that although a few species are resistant to elevated CO2, most lack sufficient resistance resulting in their decline. This has led to the simple grouping of coral species into “winners” and “losers,” but the physiological traits supporting this ecological assessment are yet to be fully understood. Here using CO2 seeps, in two biogeographically distinct regions, we investigated whether physiological traits related to energy production [mitochondrial electron transport systems (ETSAs) activities] and biomass (protein contents) differed between winning and losing species in order to identify possible physiological traits of resistance to ocean acidification and whether they can be acquired during short-term transplantations. We show that winning species had a lower biomass (protein contents per coral surface area) resulting in a higher potential for energy production (biomass specific ETSA: ETSA per protein contents) compared to losing species. We hypothesize that winning species inherently allocate more energy toward inorganic growth (calcification) compared to somatic (tissue) growth. In contrast, we found that losing species that show a higher biomass under reference pCO2 experienced a loss in biomass and variable response in area-specific ETSA that did not translate in an increase in biomass-specific ETSA following either short-term (4–5 months) or even life-long acclimation to elevated pCO2 conditions. Our results suggest that resistance to ocean acidification in corals may not be acquired within a single generation or through the selection of physiologically resistant individuals. This reinforces current evidence suggesting that ocean acidification will reshape coral communities around the world, selecting species that have an inherent resistance to elevated pCO2.

Continue reading ‘Greater mitochondrial energy production provides resistance to ocean acidification in “winning” hermatypic corals’

Acidification impedes shell development of plankton off the US West Coast

Shelled pteropods, microscopic free-swimming sea snails, are widely regarded as indicators for ocean acidification because research has shown that their fragile shells are vulnerable to increasing ocean acidity. 

A new study, published in the journal Scientific Reports, shows that pteropods sampled off the coasts of Washington and Oregon made thinner shells than those in offshore waters. Along the coast, upwelling from deeper water layers brings cold, carbon dioxide-rich waters of relatively low pH to the surface. The research, by a team of Dutch and American scientists, found that the shells of pteropods collected in this upwelling region were 37 percent thinner than ones collected offshore.

Sometimes called sea butterflies because of how they appear to flap their “wings” as they swim through the water column, fat-rich pteropods are an important food source for organisms ranging from other plankton to juvenile salmon to whales. They make shells by fixing calcium carbonate in ocean water to form an exoskeleton. 

Continue reading ‘Acidification impedes shell development of plankton off the US West Coast’

Pteropods make thinner shells in the upwelling region of the California Current ecosystem

Shelled pteropods are widely regarded as bioindicators for ocean acidification, because their fragile aragonite shells are susceptible to increasing ocean acidity. While short-term incubations have demonstrated that pteropod calcification is negatively impacted by ocean acidification, we know little about net calcification in response to varying ocean conditions in natural populations. Here, we examine in situ calcification of Limacina helicina pteropods collected from the California Current Ecosystem, a coastal upwelling system with strong spatial gradients in ocean carbonate chemistry, dissolved oxygen and temperature. Depth-averaged pH ranged from 8.03 in warmer offshore waters to 7.77 in cold CO2-rich waters nearshore. Based on high-resolution micro-CT technology, we showed that shell thickness declined by ~ 37% along the upwelling gradient from offshore to nearshore water. Dissolution marks covered only ~ 2% of the shell surface area and were not associated with the observed variation in shell thickness. We thus infer that pteropods make thinner shells where upwelling brings more acidified and colder waters to the surface. Probably the thinner shells do not result from enhanced dissolution, but are due to a decline in calcification. Reduced calcification of pteropods is likely to have major ecological and biogeochemical implications for the cycling of calcium carbonate in the oceans.

Continue reading ‘Pteropods make thinner shells in the upwelling region of the California Current ecosystem’

Addressing the impacts of multiple stressors on shellfish aquaculture through research/industry partnerships

Grant Application Deadline: Tuesday, 16 March 2021

Application Information: More information

Description: 

The National Sea Grant Office (NSGO) and the Ocean Acidification Program (OAP) are seeking applications that establish, continue, and/or expand collaborations between researchers and the shellfish aquaculture industry. Specifically, applications to this competition should utilize new or existing research/industry partnerships to study how ocean and coastal acidification in combination with other stressors impacts shellfish aquaculture. Projects should utilize multiple parameter physical, chemical, or biological observing systems and/or conduct multiple stressor experimental research. The priorities of this funding opportunity are to (1) build or strengthen relationships between the shellfish aquaculture industry and the aquaculture research community (including university, industry, private sector, tribal, state, and/or federal scientists representing diverse perspectives), (2) develop scientific knowledge on the impact of ocean and coastal acidification in combination with other stressors to shellfish aquaculture, and (3) create data products, tools, technologies, management practices, or other deliverables that are broadly applicable to building resilience within the shellfish aquaculture sector.

Subject to the availability of funding, NSGO and OAP anticipate approximately $2,000,000 total will be available to support approximately 2-6 projects, with each project funded at the approximate level of $100,000 – $300,000 per year for 1-3 years.

This document sets out requirements for submitting to NOAA-OAR-SG-2021-2006704. Additional guidance and tips on how best to prepare an application are provided in the Sea Grant General Application Guide available at (https://seagrant.noaa.gov/Portals/1/Guidance/SeaGrantGeneralApplicationGuide.pdf)

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