Marine fish survival is threatened by ocean acidification, but the hormonal mechanisms for pH compensation are not well understood, limiting mechanistic understanding of stress responses in marine fish. We examined isotocin signaling in marine medaka (Oryzias melastigma) exposed to year-2100 ocean acidification conditions (Pco2 ∼0.14 kPa, pH 7.6). Our analysis demonstrated that isotocin receptor b (ITRb) was selectively upregulated at 6 h postexposure in adult gills, though it showed only a nonsignificant trend at 5 days postfertilization (dpf) embryos, whereas adenylyl cyclase 5 (ADCY5) showed hypercapnia responsiveness primarily at hatching. Using immunofluorescence and confocal microscopy, we found that both ITRb and ADCY5 proteins localize to the basolateral membrane of Na+-K+-ATPase-positive ionocytes, partially separated from apical H+-secretion machinery. Knockdown experiments showed that ITRb-ADCY5 coupling is crucial for pH compensation, with individual knockdown moderately reducing H+ secretion and combined knockdown causing severe impairment (>70% reduction) and decreasing transcription of acid-secretion genes (nhe3, ca2, and rhcgb) by 44%–60%. Paradoxically, double knockdown triggered a twofold cAMP increase that failed to restore function, whereas wild-type embryos maintained stable cAMP levels across pH conditions, consistent with the hypothesis that ITRb-ADCY5 coupling may organize cAMP production within specific basolateral microdomains, though direct subcellular imaging would be required to validate this compartmentalization model. The developmental asynchrony between ITRb (5 dpf) and ADCY5 (hatching) responses indicates life-stage-specific vulnerabilities. Our findings reveal that basolateral ITRb-ADCY5 coupling represents a critical control point for pH compensation capacity.
NEW & NOTEWORTHY Isotocin signaling, through ITRb-ADCY5 coupling, enables marine fish to compensate for ocean acidification via compartmentalized rather than global cAMP production. Paradoxically, disrupting this pathway doubles cAMP levels yet abolishes H+ secretion, revealing that signal localization determines function. The developmental asynchrony between ITRb and ADCY5 responses suggests that pH-stress vulnerabilities are specific to each life stage and require further investigation.
Anthropogenic CO2 emissions and their continuous dissolution into seawater lead to seawater pCO2 rise and ocean acidification (OA). Phytoplankton groups are known to be differentially affected by carbonate chemistry changes associated with OA in different regions of contrasting physical and chemical features. To explore responses of phytoplankton to OA in the Chinese coastal waters, we conducted a mesocosm experiment in a eutrophic bay of the southern East China Sea under ambient (410 µatm, AC) and elevated (1000 µatm, HC) pCO2 levels. The HC condition stimulated phytoplankton growth and primary production during the initial nutrient-replete stage, while the community diversity and evenness in both pCO2 treatments were reduced during this stage due to the rapid nutrient consumption and diatom blooms, and the subsequent shift from diatoms to hetero-dinoflagellates led to a decline in primary production during the mid and later phases under nutrient depletion. HC treatment suppressed the diatom-to-dinoflagellate succession and enhanced the subsequent remineralization of organic matter, thereby facilitating smaller phytoplankton to dominant and sustaining primary production. Our findings indicate that, the impacts of OA on phytoplankton diversity in the coastal water of the southern East China Sea depend on availability of nutrients, with primary productivity and biodiversity of phytoplankton reduced in the eutrophicated coastal water.
The Southern California Bight 2023 Regional Monitoring Program’s Ocean Acidification study element has begun working to assess the effects of ocean acidification (OA) on shell-forming organisms in close coordination with two other West Coast ocean monitoring programs – a collaboration intended to promote standardization of OA biological monitoring across the West Coast and ensure OA’s effects on Southern California marine organisms can be placed into a broader regional context.
A field crew for the Southern California Bight Regional Monitoring Program lowers a pair of plankton nets into coastal waters to collect shelled marine gastropods that could serve as an indicator of ocean acidification’s biological effects. The Bight ’23 Ocean Acidification study element kicked off OA biological sampling in July in close coordination with two West Coast monitoring counterparts, paving the way for researchers to build a unified, comprehensive picture of OA status and trends. (Courtesy of Ami Latker, City of San Diego Public Utilities Department)
The California Cooperative Oceanic Fisheries Investigations (CalCOFI) and NOAA West Coast Ocean Acidification (WCOA) program began OA biological sampling in Southern California coastal waters farther offshore in July, the same time period that Bight ’23 is conducting OA biological sampling in nearshore waters. The biological sampling is being complemented by ocean chemistry measures of OA.
A key priority for the SCCWRP-facilitated Bight ’23 OA study element will be identifying a shell-forming species that can serve as an indicator of OA’s biological effects in Southern California coastal waters. During the first coordinated sampling effort in 2021 and 2022, the species that all three West Coast OA monitoring programs tracked – a tiny sea snail known as Limacina helicina – could not be found in sufficient abundance in Southern California nearshore waters to generate a reliable assessment of OA’s biological effects.
As a result, researchers have not yet been able to compare how marine organisms in nearshore Southern California waters are being affected by OA relative to what is happening West Coast-wide. Researchers’ goal is to stitch together biological data from multiple West Coast monitoring programs to create a unified, comprehensive picture of OA status and trends.
The coordinated, summer-long OA field sampling event follows years of collaborative work by the trio of monitoring programs and other partners to develop repeatable, standardized methods for tracking OA-triggered shell dissolution in shelled marine gastropods.
This method development work culminated with a one-year intercalibration exercise facilitated by SCCWRP and CalCOFI, completed in June, that standardized the process by which gastropod shell dissolution is analyzed under a scanning electron microscope. This new protocol can now be used on the samples collected by all three programs during this summer’s field sampling.
Shelled gastropods are sensitive to OA’s corrosive effects, enabling them to serve as sentinel biological indicators of the pace and intensity with which OA is unfolding across the West Coast.
To address the Limacina data gap in Southern California, all three programs have agreed to sample alternate swimming marine snail species this summer that could potentially complement Limacina monitoring. Researchers are focusing initially on Clio pyramidata – a type of sea snail known as a pteropod – and Atlanta californiensis – a heteropod species. Both are commonly found in the warmer waters of Southern California; the other two monitoring programs are examining whether these species can be found in abundance farther north as well.
Researchers envision developing a suite of shell-forming organisms to serve as sentinel biological indicators for tracking West Coast OA. Data from different biological indicators across different parts of the West Coast would enable researchers to take a multiple-lines-of-evidence approach to tracking OA’s biological effects, with a goal to enhance management understanding and confidence in OA monitoring data.
Researchers hypothesize that Southern California coastal waters may be too warm for Limacina – or at least they were during the years Southern California OA biological sampling was previously conducted. CalCOFI, which samples further offshore, successfully sampled Limacina at some offshore sites in Southern California, while the Bight program identified Limacina during the wintertime in mostly cooler, upwelling-dominated waters in the Santa Barbara Channel.
If promising alternate indicators can be identified this summer, Bight ’23 may follow up with subsequent rounds of sampling to investigate seasonal patterns in shell dissolution.
In addition to monitoring ocean chemistry and shell dissolution, the trio of programs are piloting the use of environmental DNA (eDNA)-based methods for tracking OA’s biological effects on marine life.
Biology is one of two approaches that researchers are taking to track OA in coastal waters. The other approach – tracking ocean chemistry – already has been implemented by West Coast OA monitoring programs, including the Bight program.
This summer’s coordinated OA sampling effort highlights all three monitoring programs’ shared commitment to building a comprehensive picture of West Coast OA. While the Bight program monitors OA in Southern California nearshore coastal waters, the CalCOFI and WCOA surveys are focused on monitoring OA farther offshore and northward.
An updated version of the OA-ICC bibliographic database is available online.
The database currently contains 9,928 references and includes citations, abstracts and assigned keywords. Updates are made every month.
The database is available as a group on Zotero. Subscribe online or, for a better user experience, download the Zotero desktop application and sync with the group OA-ICC in Zotero. Please see the “User instructions” for further details.
Sundarbans, the world’s largest contiguous mangrove ecosystem and representing shallow coastal Bay of Bengal of the Northern Ocean, faces dynamic climate variations, including ocean acidification. To delineate ocean acidification from natural pH variations, it is crucial to perform long-term measurements of multiple carbonate chemistry parameters such as pH, total alkalinity (TA), and dissolved nutrients, among others. In the present study, surface water carbonate chemistry parameters, including TA, pH, and dissolved nutrients (o-phosphate and silicate), were analysed monthly between 2014 and 2022 in three pre-defined stations, namely Stn1, Stn2, and Stn3, part of Sundarbans Biological Observatory Time Series (SBOTS) located in Sagar Island, the largest island of the Indian Sundarbans. The observed deviation from the linear TA-Salinity curve in the studied sites of SBOTS showed the influence of freshwater in modulating TA. Generalized Additive Model (GAM) revealed substantial seasonal variability in the controls on TA. During monsoon, salinity was a dominant driver of carbonate chemistry, consistent with enhanced freshwater discharge. In contrast, during the post-monsoon season, primary productivity as indicated by the relationship with Chla, dissolved silicate, was found to exert a stronger influence on TA variability. Multilinear regression (MLR) analysis of calculated pCO2 further supported these seasonal trends. Overall, the findings highlight the importance of season-specific assessments, highlighting the critical role of freshwater discharge in shaping estuarine carbonate dynamics. These insights are vital for predicting the vulnerability and response of mangrove estuaries under future climate change scenarios.
Ocean acidification (OA) is a major component of ongoing global environmental change, yet its biological impacts on open-ocean calcifiers remain insufficiently quantified. Here, we investigate seasonal variability in the individual test density of the planktic foraminifer Globigerina bulloides in the western North Pacific. Test density was determined using high-resolution microfocus X-ray computed tomography, enabling micron-scale structural assessment. Time-series samples collected by sediment traps moored at 150 m and 540 m at station K2 (2008–2009) reveal pronounced seasonal variability, with test density reduced by ~ 20% during winter relative to other seasons. Seasonal reductions were associated with enhanced vertical mixing and positively correlated with mixed-layer pH, carbonate-ion concentration, and temperature. Additional plankton-tow samples collected between 2010 and 2016 further support a strong linkage between carbonate chemistry and calcification intensity. Multiple regression analysis shows that carbonate-ion concentration independently explains 46.6% of the variance in test density, whereas temperature accounts for only 0.25%, indicating that carbonate-ion availability exerts a dominant control on test density. Given the ongoing decline in carbonate-ion concentration in the North Pacific (~ 0.77 µmol kg−1 yr−1), our results imply an annual decrease of ~ 2 µg mm−3 in foraminiferal test density. Continued OA may therefore reduce biogenic CaCO3 shell density, potentially weakening the efficiency of the carbonate-based biological carbon pump. This study provides quantitative field-based evidence linking seasonal carbonate chemistry to shell density variability in open-ocean calcifiers.
The present study experimentally investigates how temperature and acidification affect the polar lipid fatty acid (FA) composition of European sea bass (Dicentrarchus labrax) juveniles. Fish were reared for 92 days and fed ad libitum under four conditions: a control (CT; with natural fluctuating temperature and pH); a warming (WT, +4°C above control, and control pH), an acidification (AT, control temperature and −0.4 pH units below the control), and a combined warming and acidification treatment (WAT, +4°C and −0.4 pH units relative to the control). Results showed that warm treatments (WT and WAT) increased final weight and length similarly, whether or not combined with acidification, and that acidification alone (AT) had no effect on these variables. Polar lipid FA profiles of muscle and brain were only mildly impacted by temperature and not by acidification. Overall, the results suggest that European sea bass, under unrestricted quantity of food is able to maintain a very stable polar lipid FA composition. However, complementary studies with individuals under different feeding regimes or from wild populations subject to natural dietary variability are needed.
Cytoplasmic pH homeostasis was critical for N. oceanica to tolerate 5% CO2.
The tolerant mechanisms involved multiple physiological-biochemical processes.
H+-PPase was the central regulator for alleviating cytoplasmic acidification.
avp1 overexpression improved cytoplasmic pH regulation by raising H+-PPase activity.
Abstract
High CO2 tolerance microalgae screen/breeding shows the urgent research priority when applying microalgae for flue gas CO2 sequestration. In this study, we chose the important resource microalgae Nannochloropsis oceanica as the target organism, the regulatory mechanisms of N. oceanica were elucidated under 5% and 20% high CO2 conditions, and the function of key regulating gene avp1 encoding H+-PPase was further explored. The results showed N. oceanica was tolerant to the 5% CO2 that maintained intracellular pH homeostasis, while severe cytoplasmic acidification was occurred under the 20% CO2 condition. Integrated physiological, biochemical, and transcriptomic analysis revealed that P-ATPase and H+-PPase activities were enhanced at 4 h and 4 d under the 5% CO2 condition, respectively. Concurrently, the reprogramming of organic acid metabolism and maintenance cellular energy supply additionally mitigated intracellular acidification. Further functional validation showed that overexpression of avp1 enhanced H+-PPase activity, increased cytoplasmic pH values, promoted pigments accumulation and growth of N. oceanica under the high CO2 condition. Therefore, this study clarified the working mode of N. oceanica to tolerant high CO2 and cytoplasmic acidification, and firstly timely proved the function of avp1, provided important data basis and gene candidates for the research of applying microalgae to CO2 sequestration.
Freshwater fluxes from precipitation and river runoff play a critical role in modulating upper-ocean stratification, nutrient availability, and biogeochemical processes in the coastal waters of the Indian subcontinent. The formation of the barrier layer thickness (BLT) links freshwater input to vertical mixing, influencing both productivity and carbonate chemistry. High-resolution (5 km) MITgcm-BLINGv2 simulations are conducted for the Arabian Sea (AS) and the Bay of Bengal (BoB), and sensitivity experiments are performed to represent reduced and increased freshwater perturbations. We analyzed seasonal variability of buoyancy frequency (N2), mixed layer depth (MLD), net primary productivity (NPP), pH, and phytoplankton biomass across five coastal regions. Reduced freshwater scenarios weakened or eliminated BLT, leading to deeper MLD and N2 maxima, with subsurface nutrient-rich waters entrained upward. This enhanced nutrient availability increased NPP in the coastal regions. However, the upward transport of subsurface carbon also lowered surface pH by 0.03, indicating a trade-off between biological enhancement and increased surface acidification. In the increased freshwater scenario, the BLT strengthened, the MLD shoaled, and NPP decreased, while surface pH increased due to reduced vertical carbon exchange. Interestingly, stratification deepening under reduced freshwater input is more pronounced in the southeastern AS than in the BoB, contrasting conventional understanding. Vertical phytoplankton responses are consistent with these trends, with small and large phytoplankton biomass increasing under weaker BLT and decreasing under enhanced BLT. Freshwater-driven BLT modulation drives a complex interplay between carbon uptake and export along Indian coastal waters. These findings emphasize the importance of accurately representing freshwater fluxes in biogeochemical models to capture regional ecosystem responses.
Macroalgae are a cornucopia of bioactive molecules whose synthesis is modulated in response to environmental variables, allowing macroalgal adaptation and survival. Among these metabolites, polyphenols are worthy of attention because they may represent an informative archive of environmental conditions, given their involvement in several ecological and biochemical functions. This systematic review synthesized the literature from the last two decades on phenolic compounds in Mediterranean macroalgae, focusing on their functional roles, taxonomic distribution, geographical occurrence, and responses to abiotic and biotic stressors, with particular attention to their potential as ‘early indicators’ of environmental change. Using a Scopus-based search strategy, nearly 6000 records were screened and studies on brown, red, and green macroalgae published between 2003 and September 2025 across the Mediterranean basin were retained.
The reviewed literature highlighted that polyphenols are widely involved in antioxidant defense, photoprotection, stress tolerance, and interspecific interactions, supporting the resilience of macroalgae in a rapidly changing Mediterranean Sea. At the same time, the review emphasizes the significant biotechnological potential of these compounds in the pharmaceutical, cosmetic, food, and agricultural sectors. Overall, the evidence indicates that fluctuations in polyphenol content often reflect rapid physiological responses to environmental stress, suggesting that these compounds may function as early indicators of ecological change in Mediterranean macroalgae.
Despite growing evidence, several important gaps remain. Research from the eastern Mediterranean basin is still limited, most studies focus on only a few model genera, and long-term field studies examining multiple environmental stressors are rare. Future research should therefore include a wider range of geographic areas and species, adopt standardized analytical methods, and investigate how polyphenols respond to combined environmental drivers. This would help clarify and validate their use as early indicators of ecological change.
Nutrient limits the phytoplankton growth in the Bay of Bengal due to stratification.
Picoplankton dominated the phytoplankton community in the Bay.
An increase in phytoplankton biomass was observed in response to ocean acidification (OA) in nutrient-rich regions.
Decrease in phytoplankton biomass, bacteria and microzooplankton abundance was found in the nutrient-poor regions.
Nutrient limitation overshadow the ocean acidification impact in the Bay of Bengal.
Abstract
The perennial rise in atmospheric carbon dioxide (CO2) from anthropogenic activities increased oceanic carbon uptake, thereby lowering seawater pH. Rapid ocean acidification (OA) by 2 to 3 times than other regions of the same latitudinal belts was reported in the Bay of Bengal (BoB) due to the deposition of atmospheric pollutants, in addition to the dissolution of atmospheric CO2. Therefore, the impact of OA on phytoplankton composition is hypothesized to be greater in the BoB than elsewhere in the globe. On the other hand, the BoB experiences intense oligotrophic conditions due to strong stratification driven by nutrient-poor freshwater discharge from major rivers, which is the primary bottleneck for phytoplankton growth, rather than carbon. It is hypothesized that nutrient limitation may overshadow OA’s impact on plankton in the BoB. To test this hypothesis, six microcosm experiments were conducted in the western BoB between 13 and 20°N with different nutrient levels in the surface waters. The pH of the surface water was adjusted by 0.2 units below the ambient pH using CO2 gas bubbling to simulate the projected OA scenario for the year 2100. Following pH adjustment, incubations were carried out for three days. Picoplankton dominated the phytoplankton community (∼96%) at all stations, with prochlorophytes as the dominant group. Among the phytoplankton, procholorophytes, diatoms, and prymeniophytes increased due to OA across all stations, while other groups of phytoplankton were declined. The increase in phytoplankton biomass was observed in the nutrient-rich stations, while a decrease in phytoplankton biomass, bacteria and microzooplankton abundance was found in the nutrient-poor stations, suggesting that nutrient availability dominated over the OA. Under a business-as-usual scenario, an increase in stratification due to melting Himalayan glaciers is projected, leading to strong stratification and reduced nutrient inputs through vertical mixing in the future in the BoB. Under such a scenario, nutrient limitation may possibly overshadow the OA impact in the future, despite a rapid decline in pH driven by increased CO2 dissolution and deposition of atmospheric pollutants.
Plankton community respiration (PCR) plays a central role in aquatic ecosystems, driving the breakdown of organic matter and influencing global carbon cycling through its contribution to the production and consumption of carbon and oxygen. Coastal areas are regarded as metabolic hotspots in the oceans, due to their intense biological and biogeochemical activities. This review synthesizes experimental evidence to explore how environmental constraints and climate drivers affect PCR in European coastal waters. In total, 46 studies were found in which PCR was measured during experiments testing the effects of one or multiple global climate change drivers in European coastal waters. Among them, the majority of experiments focused on changes in temperature, nutrient concentrations and stoichiometry, and/or pH, while other stressors were less studied. In addition to this qualitative synthesis, a quantitative meta-analysis was conducted on warming and acidification experiments, the only drivers for which comparable experimental designs were available. This analysis, based on 19 warming and 6 acidification studies, was used to perform a standardized comparison of effect sizes across studied areas and experimental set-ups. This review highlights critical knowledge gaps, notably regarding non- and understudied areas and understudied interactions between stressors that occurs jointly in ecosystems.
NOAA’s West Coast Ocean Acidification survey is a long-running effort with previous missions like this one aboard the NOAA Ship Ronald H. Brown in 2021 seen near an ocean acidification monitoring mooring. Credit: NOAA
Today, researchers set sail for a seventh West Coast Ocean Acidification (WCOA 2026) research mission. Departing from San Diego, CA aboard the University of Alaska, Fairbanks R/V Sikuliaq, the team will travel north to Washington as part of NOAA’s efforts for long-term monitoring ocean acidification and its impacts on marine resources.
This intensive 30-day coastal research cruise faces the unique opportunity of sampling during conditions building toward an extreme El Niño. El Niño brings warmer water and can alter upwelling and precipitation patterns in the region. If it forms as predicted, sea surface temperatures may rise 2℃ (3.6℉) or more above average. Phytoplankton and zooplankton communities would follow these warmer waters, which would impact food webs and fisheries as seen during past marine heatwaves. WCOA 2026 will couple ocean chemistry, biology, and physics to build a more comprehensive view of how extreme events impact biogeochemistry and ecosystems.
Data collected during WCOA 2026 are essential for validating models and forecasts of ocean conditions like J-SCOPE. This short term (six to nine month) forecast is used for the California Current Integrated Ecosystem Assessment. High quality ocean carbon chemistry data is required to forecast the saturation state of calcium carbonate, the mineral building block used by shellfish like Dungeness crabs, clams, and oysters.
“Results from past WCOA cruises have provided novel insights into acidification rates and impacts in coastal and estuary habitats that provide our nation with rich fish and shellfish resources,” says Co-Chief Scientist Dr. Simone Alin from PMEL. She also notes that “these findings have also contributed to innovative tools and information products that support healthy fisheries, ecosystems, and communities.” Resource managers and harvesters can use this information to inform fisheries management decisions.
Other coordinated regular coastal fisheries surveys, including the California Cooperative Oceanic Fisheries Investigations (CalCOFI) enhance the impact of WCOA 2026. By conducting biological net tows at established stations, researchers will gain high-quality data on marine life.
Ultimately, the data collected through WCOA 2026 will serve as a foundational benchmark for monitoring, modeling and research in the region. These data will provide calibration information for sensor networks designed to fill measurement gaps between research cruises. With the potential for sampling to occur as ocean conditions change with El Niño, NOAA and our partners will be able to better understand how extreme conditions alter vital marine resources like Dungeness crabs, krill, plankton and other fisheries. The mission concludes on July 16th.
The IAEA’s Ocean Acidification International Coordination Centre (OA-ICC) (https://www.iaea.org/ocean-acidification) is cooperating on the organization of this event and has limited funds at its disposal to help meet the costs of the attendance of selected participants from eligible IAEA Member States to present their work and foster international collaboration at the conference.
The deadline to apply is Friday September 4th. To apply, applicants will need to submit their application through their national authority to the IAEA – in most cases, this will be your country’s permanent mission to the IAEA.
If you have questions on the application procedure, please contact Ms Lina Hansson (L.Hansson(at)iaea.org) or Ms Carolina Galdino (C.Galdino(at)iaea.org).
Dates: 13–16 October 2026
Location: Tākina Convention & Exhibition Centre, Wellington, New Zealand
Introduction Held every four years, the International Symposium on the Ocean in a High-CO2 World is the largest global gathering on ocean acidification. The Symposium brings together scientists, communities, policymakers, and stakeholders from sectors such as fisheries and aquaculture to share and discuss the latest research on the impacts of ocean acidification on marine life and potential solutions to counter its effects. The Sixth edition will maintain a focus on ocean acidification but will also expand to consider other ocean climate impacts, such as ocean warming and deoxygenation, particularly in a multiple-driver context. Research on marine carbon dioxide removal interventions, which have been suggested to mitigate climate change and ocean acidification, will also be a key part of the conference. More information on the seven key themes of the conference, its 22 workshops, and the detailed programme can be found on the Symposium website (https://highco2-vi.org/).
Objectives The purpose of the event is to provide a space for scientists from the ocean acidification (OA) research community to disseminate the results of their research, discuss joint projects with peers and engage with stakeholders. The event, held every four years, is the world’s largest conference on OA and related stressors.
Target Audience Scientists from developing IAEA Member States. Priority will be given to early-career scientists actively working on ocean acidification, multiple stressors or marine carbon dioxide removal (mCDR). Applicants should hold a university degree in marine biology, oceanography or a related scientific field. Female scientists are encouraged to apply.
Working Language English
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Participation and Registration All persons wishing to participate in the event through the IAEA have to be designated by an IAEA Member State.
In order to be designated by an IAEA Member State, participants are requested to send the Participation Form (Form A) and Grant Application Form (Form C – if applicable) to their competent national authority (e.g. Ministry of Foreign Affairs, Permanent Mission to the IAEA or National Atomic Energy Authority) for onward transmission to the IAEA by 4 September.
Selected participants will be informed in due course on the procedures to be followed with regard to administrative and financial matters.
Participants are hereby informed that the personal data they submit will be processed in line with the Agency’s Personal Data and Privacy Policy and is collected solely for the purpose(s) of reviewing and assessing the application and to complete logistical arrangements where required. The IAEA may also use the contact details of Applicants to inform them of the IAEA’s scientific and technical publications, or the latest employment opportunities and current open vacancies at the IAEA. These secondary purposes are consistent with the IAEA’s mandate.
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Additional Requirements The participants should have a university degree in marine chemistry, biology, oceanography, or a related scientific field, and must be active researchers in the ocean acidification /mCDR field or should have already received technical training on ocean acidification / mCDR by the IAEA or through other efforts.
Selection will be based on merit and motivation. Applications must include, in addition to Form A and C, if applicable:
A motivation letter with a short description of research interests, motivation to participate, and plans regarding present and future research on OA and/or mCDR (max one A4 page)
High carbonate alkalinity can impose composite hydrochemical stress on bivalves by altering inorganic carbon speciation, buffering capacity and ion-exchange conditions. The Hong Kong oyster (Crassostrea hongkongensis) naturally inhabits estuarine areas where low salinity can occur together with carbonate-system disturbance, yet its physiological response to high carbonate alkalinity under a low-salinity background remains unclear. In this study, oysters were exposed at salinity 5 to a Control treatment, A10 (carbonate alkalinity 10 mmol L⁻¹) and A20 (carbonate alkalinity 20 mmol L⁻¹). Salinity was treated as a controlled background condition, whereas the alkalinity treatments represented a composite carbonate-alkalinity perturbation involving alkalinity, pH and carbonate chemistry. Survival, feeding rate, oxygen consumption, ammonia excretion and hemolymph ammonia were measured across Control, A10 and A20. Hemolymph catalase (CAT), glutathione peroxidase (GSH-Px), glucose and ATP were analyzed as A10 and A20 time-course endpoints because a parallel Control was not included for these assays. A 24-h mantle transcriptome was used to screen candidate transcripts associated with innate defense and glutathione metabolism. Oysters under A10 retained partial compensatory capacity, whereas A20 significantly reduced survival. A10 and A20 suppressed early oxygen consumption and ammonia excretion, and altered feeding allocation among microalgae. Within the A10 and A20 biochemical time-course dataset, CAT, GSH-Px, glucose and ATP showed stage-specific variation, suggesting redox and energy-metabolism adjustment under alkalinity stress. Overall, high carbonate alkalinity reduced survival and was associated with metabolic depression, feeding compensation and hemolymph redox-energy remodeling in C. hongkongensis. This study will provide a theoretical basis for water quality regulation in saline-alkaline water aquaculture and the sustainable development of aquaculture.
Marine diatoms are key components of the planetary ocean, playing crucial roles in trophic networks: fixing carbon, producing fatty acids (FA) that cannot be synthesized de novo by higher trophic organisms. To better comprehend how the combined action of climatic change influences the dietary value and the impacts on the upper trophic levels, we used a 2 × 2 factorial experiment to investigate how the FA profiles of Skeletonema marinoi changed in response to the individual and combined effects of warming (7, 19°C) and acidification (400, 1 000 ppm CO2). Three S. marinoi strains were exposed for ~ 40 weeks to ambient conditions, warming, acidification and their combination and analyzed for FA profiles, focusing on polyunsaturated (PUFA), omega-3 and omega-6 FA. We found that omega-3 FA increased under warming exposure, while acidification alone led to lower omega-3/omega-6 ratios. In contrast, the ratios increased under warming, alone or coupled with acidification, in all three strains, indicating better food quality for higher trophic levels. Our results suggest the long-term exposure to both drivers will help the marine diatoms to acclimatize to their combined effects, allowing them to buffer the changes brought by warming and ocean acidification.
Marine bivalves play pivotal ecological and economic roles but are increasingly challenged by multiple environmental stressors. Although there is extensive research on the effects of individual stressors, a comprehensive review is needed to synthesize current evidence and clarify how multiple interacting stressors collectively affect the physiology and resilience of marine bivalves. This review integrates evidence from 2010 to 2025, encompassing 178 peer-reviewed studies emphasizing the combined and interactive impacts of climate change drivers (warming, hypoxia, salinity, and ocean acidification), pollutants (heavy metals, persistent organic pollutants, endocrine-disrupting chemicals, antibiotics, nanoparticles, microplastics), and microbiome shifts on future aquaculture resilience. Literature was systematically retrieved from Web of Science, Scopus, PubMed, and Google Scholar in accordance with PRISMA 2020 guidelines. Bibliometric mapping (VOSviewer 1.6.20) revealed a rapid growth in research after 2018, characterized by studies on Mytilus, Crassostrea, and Ruditapes. A systematic evaluation of recent evidence was conducted, combining data from physiological, molecular, and microbial studies, with particular attention to implications for aquaculture. The analysis reveals that stressors rarely act alone. Instead, their cumulative and interactive effects cause oxidative stress, disrupted energy allocation, destabilized host-microbe relationships, lowered tolerance thresholds, and other eco-physiological consequences. These results highlight the vulnerability of bivalve populations to rapid coastal urbanization, declining water quality, and sediment contamination. The review concludes that resilience can be enhanced through selective breeding for stress-tolerant genotypes, integrated monitoring of pollutants and microbial indicators, and multi-omics approaches to guide adaptive aquaculture management.
We innovatively define two biophysical parameters (pH change rate and acceleration) of Zostera japonica photosynthesis.
pH change acceleration is sensitive to the regulation of carbon utilization pathways during Z. japonica photosynthesis.
A progressive pH–salinity-driven regulation mode of carbon utilization pathways in Z. japonica photosynthesis is summarized.
The impact of ocean acidification and alkalization on seagrass is characterized from a new biophysical standpoint.
Abstract
Seagrass carbon concentration mechanisms are modulated by seawater pH and salinity, yet their progressive regulation in photosynthetic inorganic carbon utilization pathways remain poorly characterized. This study novelly mathematically characterized first-order (pH change rate) and second-order (pH change acceleration) derivatives from pH-drift experiments in the intertidal seagrass Zostera japonica along China’s coastline. The pH change-based method effectively highlights the dissolved inorganic carbon (DIC) utilization and biomass accumulation of seagrass, while pH change acceleration is sensitive to the progressive switch of DIC utilization pathways during Z. japonica photosynthesis. As pH increases, six significant regulations emerge that have ecophysiological significance: a. primary regulation via reaching the CO2 compensation point; b. extracellular carbonic anhydrase (exCA) activity dropping to negligible levels; c. diminished efficiency of proton pump-mediated extracellular acidification in supplying CO2; d. regulation via reaching the bicarbonate saturation point; e. regulation via reaching the bicarbonate compensation point; and f. regulation via enhanced respiratory CO2 into seawater temporarily stimulating photosynthesis as a feedback. We summarize a progressive and universal pH–salinity-driven regulation mode reflecting different combinations of DIC utilization pathways and their respective intensities. Specifically, seawater pH modulates the mode’s fluctuating thresholds, while salinity governs the amplitude. Unexpectedly, elevated salinity serves as an effective stimulant for Z. japonica to maintain strong DIC utilization intensity in high pH (> 9.1) environments. Ocean acidification could increase pH change acceleration by 166%, enhancing carbon fixation, whereas artificial ocean alkalinity enhancement (Ca(OH)2 supplementation and olivine-seawater weathering) could reduce it by 165% and 105%, respectively, risking mortality of Z. japonica. Additionally, the salinity-dependent braking point (where acceleration drops to zero) serves as a critical threshold for seagrass photosynthesis and a new factor for transplantation-based restoration.
This study investigated the spatial distribution, seasonal variation, and drivers of surface seawater alkalinity (Alk) in Zhanjiang Bay (ZJB) using high-frequency seasonal sampling in the summers and winters of 2023. Surface Alk ranged from 525.3 to 2213.3 μmol·L−1, with mean values of 1373.1 ± 420.9 μmol·L−1 (summer, n = 28) and 1612.3 ± 343.7 μmol·L−1 (winter, n = 20). Spatially, Alk increased progressively from the estuary to the inner bay and further to the bay mouth, reflecting a typical dilution gradient. Correlation analyses showed that summer Alk was positively correlated with salinity (ρ = 0.706, p < 0.001), indicating that salinity changes associated with conservative mixing were a dominant control, whereas the weaker winter correlation (ρ = 0.473, p < 0.001) suggested that biological processes may play a more important role. Tidal forcing was significantly associated with diurnal Alk variations, particularly in the estuary and inner bay. In the estuary, high Alk occurred during high tide, consistent with tidal mixing; in the inner bay, elevated Alk was observed during low tide, suggesting a possible tidal pumping effect. These findings provide baseline data on Alk dynamics in a subtropical estuarine bay and contribute to understanding the carbonate system and buffering capacity in similar coastal systems. However, because measurements of dissolved inorganic carbon and pCO2 were unavailable, a quantitative assessment of carbon sink capacity requires further investigation.
Amidst global climate change, the escalating atmospheric CO2 levels have intensified ocean acidification (OA), significantly impacting the structure and function of marine ecosystems. Seagrass beds, representative nearshore ecosystems, play a pivotal role in carbon sequestration, biodiversity preservation, and nearshore environmental equilibrium. Rhizosphere microorganisms within seagrass beds, essential components of the ecosystem, drive material cycling and energy flow. Their community structure and functions demonstrate heightened sensitivity to environmental variations. While previous studies have primarily focused on the effects of ocean acidification on seagrass hosts, limited attention has been given to the rhizosphere. Therefore, this study selected Zostera japonica as the focal species and systematically evaluated changes in the structure and function of the rhizosphere bacterial community across varying acidification levels (400 ppm, 1,000 ppm, 2,000 ppm CO2) within an ocean acidification context. The results revealed a significant decline in the richness and diversity of the rhizosphere bacterial community under acidification, accompanied by shifts in community composition characterized by an increase in the relative abundance of Bacteroidota and Tenacibaculum with escalating acidification levels. In high acidification conditions, bacterial network interactions exhibited a trend toward simplification; yet the number of key taxonomic units increases, and there was a shift in community assembly from stochastic to deterministic processes. Functional predictions indicated the enhancement of microbial carbon sequestration and nitrogen fixation under acidification, while denitrification and specific sulfur metabolism pathways were inhibited. This implies that in acidified environments, the rhizosphere bacterial community may enhance carbon and nitrogen fixation to uphold nutrient supply.
Importance
Against the background of escalating global climate change and ocean acidification, seagrass beds, as crucial blue carbon sink ecosystems, face formidable challenges to their ecological functions and stability. Rhizosphere microorganisms of seagrasses, serving as the “second genome” of the seagrass host, play a central role in material cycling, nutrient supply, and system stability within seagrass beds. They are a key biological component that supports seagrass adaptation to environmental changes. Therefore, investigating the response and adaptation mechanisms of seagrass rhizosphere bacterial communities under ocean acidification is essential for deepening our understanding of the stability and resilience of seagrass bed ecosystems.
