Ocean Acidification

Scleractinian (stony) corals can build highly ordered aragonite (CaCO₃) exoskeletons, which are vital for marine ecosystems, coastal stability, and of cultural and economic importance through supporting fisheries and tourism. However, they face multiple challenges through global climate change, unsustainable human activity, and coral-specific diseases. In the latter category, Stony Coral Tissue Loss Disease (SCTLD) has recently emerged as one of the most destructive coral diseases, spreading rapidly and causing widespread tissue mortality across many marine species [1]. We have recently started exploring remnant effects of this disease on Montastraea cavernosa exoskeletons, abundant throughout the Caribbean Sea, Gulf of Mexico, and Atlantic Ocean. Indeed, through use of multi-scale electron diffraction characterization techniques, we find that their skeletogenisis is impacted by this disease from the micro- through atomic scale [2]. Notably, at the nanoscale we observe pockets of more soluble amorphous calcium carbonate (ACC) within centers of calcification (CoCs; i.e. the center of the three-dimensional fans containing arranged elongated aragonite crystals) for healthy corals, which appear absent in STCLD-afflicted corals. At the atomic level, we reveal planar defects in diseased coral, which are much lower in density in healthy corals, presumably inflicted through dysregulation processes after tissue death.

Yet, the most severe threat to global coral reefs and their exoskeletons is climate change. As oceans increase the uptake of anthropogenic CO₂ primarily from burning fossil fuels, ocean acidity has increased. The reduction in pH because of this Ocean Acidification (OA) not only reduces the rate of net ecosystem calcification, but also increases net dissolution of skeletons. At current trends, most coral exoskeletons are expected to dissolve starting in 2050 [3]. Currently, it is unclear how exactly (the onset of) dissolution proceeds and affects their aragonitic framework. Given our expertise in characterization of coral skeletons, herein we discuss a developed in-situ platform to investigate OA effects at the nanoscale.

As proof of concept, we sandwiched crushed geological aragonite nanoparticles between own fabricated SiN_x-based chips, compatible with a Protochips Atmosphere gas cell holder [4]. Thereafter, we introduced water vapor at 14 Torr at room temperature (∼60% relative humidity) for 10 minutes to create a hydrated environment for the particles (Fig. 1b). In a third step, we introduced gaseous CO₂ at a pressure of 1 atm (Fig. 1c). The formed unstable carbonic acid produces HCO₃^– and H⁺, which increases acidity (i.e. lowers pH). Indeed, we observe rapid dissolution of aragonite particles after CO₂. While we expect roughly a pH ∼4 in this system, this experimental observation matches theoretical expectations that aragonite would dissolve under these conditions (Fig 1c-f). We further observe nucleation and growth of new particles in a dendritic fashion in the vicinity of the dissolved particles (Fig 1c-f). Likely, this is crystallization of calcite, the most stable crystalline polymorph of CaCO₃, induced by local dissolution of aragonite [5]. Although pCO₂ in the oceans is expected to be much lower (∼400 – 500 µatm) and thus dissolution timescales are expected to shift, this illustrates our platform can capture aragonite dissolution.

To expand our platform methodology, using conventional Ga⁺ FIB-methods, we prepared a lamella of geological aragonite with a thinned region (∼100 nm), which was then transferred on top of a SiN_x-based chip, and attached this in one of the corners between the Si support and the SiN_x layer (Fig. 2a). We were able to sandwich the lamella between both chips when observing the cell in the TEM (Fig 2b,c). Next, we will target healthy and STCLD-afflicted coral sections to investigate how nanoscale dissolution proceeds at/near more soluble areas including defects due to the devastating OA process. This understanding may allow for more accurate forecasting of marine ecosystem collapse, enabling targeted mitigation strategies, protecting food supplies, and predicting climate feedback loops [6].

Fig. 1. In-situ OA platform experiment showing TEM snapshots of: a) Initial geological aragonite nanoparticles. Inset: selected area diffraction pattern indicating aragonite spots. b) Introduction of water vapor (14 Torr) after 10 min. The white arrow indicates an apparent hydration layer surrounding the large particle. c) Introduction of CO₂ at 1 atm pressure after 10 s. d) 60s e) 90s and f) 150s. White arrows in c-f) mark the outer layer of the large particle dissolving in time, red arrows illustrate growth of new crystals.

Fig. 2. a) SEM image of geological aragonite lamella attached to SiN^x chip. b) TEM image of lamella after cell assembly (top and bottom chip). c) In-situ selected area diffraction pattern of the lamella.

Smeets P., Wen Y., Cheng Y. & Hu X., 2026. Elucidating impact of ocean acidification on coral exoskeletons using an in-situ (S)TEM platform. Microscopy and Microanalysis. 32(Supplement_1): ozag053.708. doi: 10.1093/mam/ozag053.708. Article.