Recent studies identify ocean warming and acidification as major drivers of ecological change in the Eastern Mediterranean, posing serious threats to marine biodiversity, particularly for sessile or low-mobility organisms that cannot escape unfavourable conditions. At the same time, the need for standardised experimental approaches capable of generating high-quality data on organismal responses to multiple climate stressors has become increasingly evident. This manuscript presents a fully detailed and replicable experimental framework for simulating ocean warming and acidification in benthic marine invertebrates under controlled laboratory conditions. Detailed protocols include the technical set-up, experimental design, selection of climate scenarios, monitoring procedures and criteria for species selection and demonstrating its application through a validation case study from the MACCIMO project.
Chatzinikolaou E., Grigoriou P., Anastasiadis A., Vernadou E. & Dailianis T., 2026. A standardised experimental setup for simulating ocean warming and acidification in benthic marine invertebrates. Biodiversity Data Journal 14: e185047. doi: 10.3897/BDJ.14.e185047. Article.
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