The dose makes the poison: non-linear behavioural response to CO2-induced aquatic acidification in zebrafish (Danio rerio)

Highlights

  • Elevated CO2 altered behaviour in zebrafish but not in an additive manner.
  • Acclimations to ~900, 2200, and 4200 μatm cause increased, normal, and decreased anxiety-like behaviour.
  • Exploratory behaviour was not affected by any CO2 treatment.
  • Elevated CO2 to ~4200 μatm decreased locomotion.

Abstract

CO2-induced aquatic acidification is predicted to affect fish neuronal GABAA receptors leading to widespread behavioural alterations. However, the large variability in the magnitude and direction of behavioural responses suggests substantial species-specific CO2 threshold differences, life history and parental acclimation effects, experimental artifacts, or a combination of these factors. As an established model organism, zebrafish (Danio rerio) can be reared under stable conditions for multiple generations, which may help control for some of the variability observed in wild-caught fishes. Here, we used two standardized tests to investigate the effect of 1-week acclimatization to four pCO2 levels on zebrafish anxiety-like behaviour, exploratory behaviour, and locomotion. Fish acclimatized to 900 μatm CO2 demonstrated increased anxiety-like behaviour compared to control fish (~480 μatm), however, the behaviour of fish exposed to 2200 μatm CO2 was indistinguishable from that of controls. In addition, fish acclimatized to 4200 μatm CO2 had decreased anxiety-like behaviour; i.e. the opposite response than the 900 μatm CO2 treatment. On the other hand, exploratory behaviour did not differ among any of the pCO2 exposures that were tested. Thus, zebrafish behavioural responses to elevated pCO2 are not linear; with potential important implications for physiological, environmental, and aquatic acidification studies.

Hamilton T. J., Radke N. H., Bajwa J., Chaput S. & Tresguerres M., 2021. The dose makes the poison: non-linear behavioural response to CO2-induced aquatic acidification in zebrafish (Danio rerio). Science of The Total Environment 778: 146320. doi: 10.1016/j.scitotenv.2021.146320. Article.

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