Ocean acidification (OA) effect on various marine organisms, including shell forming bivalves, has been the subject of numerous studies in the past decade. Most of those studies have been focusing on measuring or predicting the developmental, physiological, and ecological consequences. However, OA stress produced in marine organisms is first reflected at cellular level due to altered expression of OA-responsive genes (transcriptome), proteins (proteome), bioactive peptides (peptidome), metabolites (metabolome), and then finally on phenotype. OA studies may take advantage of studying expression pattern of these genes and their products such as proteins, their modifications including “bioactive” small peptides, in response to OA-induced stress. Differentially expressed genes, proteins, and peptides in response to OA may then be used as potential biomarkers for early detection of OA symptoms. Identification of OA-responsive markers is important for climate change friendly aquaculture of bivalves such as oysters and mussels. Development of genome sequencing, mass spectrometry–based proteomics and bioinformatics tools for multiomics analyses revealed molecular response of several bivalves to OA. This chapter discusses those results and identifies knowledge gaps with an emphasis on potential application of proteomics technologies in the identification of molecular mechanisms underpinning OA effects in various marine organisms in the era of genomics and multiomics.
Thiyagarajan V., Rajan K. C., Yu Z., Qian J. & Wang Y., 2024. Ecophysiology and ocean acidification in marine mollusks: from molecule to behavior. In: Wang Y. (Ed.), Chapter 7 – Proteomic analysis of ocean acidification stress in bivalves: a synthesis and implications for aquaculture, pp. 225-264. Elsevier: Academic Press. Chapter (restricted access).
