The growth parameters and spawning behaviour of zebrafish in response to CO2 acidification demonstrated differential results. The growth performance of zebrafish is determined by key indices, BWG, SGR, CF and CV. BWG shows subtle gain in 1500 µatm group (0.09 g) and a slight decrease in 2200 µatm group (0.056 g). SGR index showed similar pattern of results, whereas CF showed a gradual decrease. The other growth index CV again showed an increase in 1500 µatm group and slight decrease in 2200 µatm group in comparison to the control group. A significant decrease in the performance of spawning behaviour was observed. At 96 hpf, the survival rate of the embryos showed a significant hit and the number of dead embryos increased dose dependently. The embryos exposed to CO2 showed a decrease in hatching rate with the increase in dose of CO2. The CO2 acidification causes notable changes in the growth and significant effect on reproductive behaviour.
Thomas A. & Kumar B., 2023. Analysis of spawning behaviour and growth indices of zebrafish in response to CO2 acidification. Journal of Coastal Life Medicine 10: 06–16. Article.