Archive for the 'Methods' Category

OA action plan toolkit & guide

The International Alliance to Combat Ocean Acidification (OA Alliance) brings together governments and organizations from across the globe dedicated to taking urgent action to protect coastal communities and livelihoods from the threat of ocean acidification and other climate- ocean impacts.

Facilitated through the OA Alliance, national, subnational, regional and tribal governments are proactively responding to the impacts of ocean acidification as they create OA Action Plans to effectively promote solutions and advancing knowledge into action.

The OA Alliance has created this Action Plan Toolkit as a guide. The OA Action Toolkit contains both regulatory and non-regulatory actions that members might consider when crafting their own OA Action. Not all OA Action plans will have the same framework or structure, as there is no “one- size fits all” approach.

Some members may choose to write a stand-alone plan, while others may decide to integrate ocean acidification mitigation, adaptation and resiliency actions across existing Climate Action Plans, Ocean Action Plans, Biodiversity or Resilience Goals and Targets, Nationally Determined Contributions pursuant to the Paris Climate Agreement, or decide to integrate and strengthen actions across other applicable management tools.

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How to measure pHT in biological experiments

Research on ocean acidification requires following best practices. The OA-ICC contributes to the development of teaching material for the implementation of simplified methodologies for laboratories with limited finances or infrastructure.

Authors: Sanja Grđan, University of Dubrovnik & Sam Dupont, University of Gothenburg

Translation: Celeste Sánchez Noguera (Spanish) and Sam Dupont (French)

Description: Measuring pH in seawater using a glass electrode is not trivial and requires TRIS buffer. TRIS buffers are commercially available from Dr. Andrew Dickson’s laboratory at the Scripps Institution of Oceanography, California. However, access to this buffer can be difficult due to a continuously increasing demand as well as costs including shipping, customs fees, and taxes, making them less available for countries and laboratory with limited funds.

A simplified buffer preparation method is described in Paulsen & Dickson (2020) making the use of TRIS buffers available to a wider range of researchers.

The aim of this document and associated material (xls sheets and videos) is to help experimentalists entering the field of ocean acidification to make their own TRIS buffer, calibrate their glass electrodes for pH measurement on the total scale, take water samples and calculate pH on the total scale (pHT).

English Language Materials

French Language Materials

Spanish Language Materials

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